Validation Data Gallery

Filter: AllWBIHC

WB analysis of A549 using 10840-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 10840-1-AP (RAP1B antibody) at dilution of 1:300. .
WB analysis using 10840-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10840-1-AP (RAP1B antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.
WB analysis using 10840-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10840-1-AP (RAP1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 10840-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10840-1-AP (RAP1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of C6 using 10840-1-AP
C6 cells were subjected to SDS PAGE followed by western blot with 10840-1-AP (RAP1B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 10840-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 10840-1-AP (RAP1B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 10840-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 10840-1-AP (RAP1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human oesophagus cancer using 10840-1-AP
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10840-1-AP (RAP1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human oesophagus cancer using 10840-1-AP
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10840-1-AP (RAP1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

RAP1B Polyclonal antibody

RAP1B Polyclonal Antibody for WB, IHC, ELISA

Cat No. 10840-1-AP

Publications(21)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

GTP binding protein smg p21B, K REV, RAL1B, RAP1, RAP1B, Ras related protein Rap 1b

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (21)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, mouse brain tissue, A549 cells, C6 cells, A431 cells, human brain tissue, HepG2 cells, mouse kidney tissue, NIH/3T3 cells
Positive IHC detected in	human oesophagus cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 20 publications below
IHC	See 1 publications below
IF	See 1 publications below
IP	See 1 publications below

Product Information

10840-1-AP targets RAP1B in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, chicken
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1282 Product name: Recombinant human RAP1B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-184 aa of BC000176 Sequence: MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDAQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTDDVPMILVGNKCDLEDERVVGKEQGQNLARQWNNCAFLESSAKSKINVNEIFYDLVRQINRKTPVPGKARKKSSCQLL 相同性解析による交差性が予測される生物種
Full Name	RAP1B, member of RAS oncogene family
Calculated molecular weight	21 kDa
Observed molecular weight	21 kDa
GenBank accession number	BC000176
Gene Symbol	RAP1B
Gene ID (NCBI)	5908
RRID	AB_2253539
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P61224
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

RAP1B is a member of the RAS-like small GTP-binding protein superfamily. Members of this family regulate multiple cellular processes including cell adhesion and growth and differentiation. RAP1B localizes to cellular membranes and has been shown to regulate integrin-mediated cell signaling. It also plays a role in regulating outside-in signaling in platelets.

Protocols

Product Specific Protocols
IHC protocol for RAP1B antibody 10840-1-AP	Download protocol
WB protocol for RAP1B antibody 10840-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Mol Cell Macrophage-Associated PGK1 Phosphorylation Promotes Aerobic Glycolysis and Tumorigenesis. Authors - Yajuan Zhang View Article
human	WB	Oncotarget LncRNA MT1JP functions as a tumor suppressor by interacting with TIAR to modulate the p53 pathway. Authors - Lihui Liu View Article
human	WB	Int J Mol Sci Molecular and Pharmacological Characterization of the Interaction between Human Geranylgeranyltransferase Type I and Ras-Related Protein Rap1B. Authors - Sonja Hinz View Article
chicken	WB,IP	Microbiol Spectr Avian Hepatitis E Virus ORF2 Protein Interacts with Rap1b to Induce Cytoskeleton Rearrangement That Facilitates Virus Internalization. Authors - Beibei Zhang View Article
human	WB	Am J Cancer Res Circ_0000231 promotes paclitaxel resistance in ovarian cancer by regulating miR-140/RAP1B Authors - Jiao Liu View Article
human	WB,IF	Viruses ICP4-Associated Activation of Rap1b Facilitates Herpes Simplex Virus Type I (HSV-1) Infection in Human Corneal Epithelial Cells Authors - Beibei Zhang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of A549 using 10840-1-AP

WB analysis using 10840-1-AP

WB analysis using 10840-1-AP

WB analysis of mouse brain using 10840-1-AP

WB analysis of C6 using 10840-1-AP

WB analysis of human brain using 10840-1-AP

WB analysis of A431 using 10840-1-AP

IHC staining of human oesophagus cancer using 10840-1-AP

IHC staining of human oesophagus cancer using 10840-1-AP

RAP1B Polyclonal antibody

RAP1B Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Macrophage-Associated PGK1 Phosphorylation Promotes Aerobic Glycolysis and Tumorigenesis.

LncRNA MT1JP functions as a tumor suppressor by interacting with TIAR to modulate the p53 pathway.

Molecular and Pharmacological Characterization of the Interaction between Human Geranylgeranyltransferase Type I and Ras-Related Protein Rap1B.

Avian Hepatitis E Virus ORF2 Protein Interacts with Rap1b to Induce Cytoskeleton Rearrangement That Facilitates Virus Internalization.

Circ_0000231 promotes paclitaxel resistance in ovarian cancer by regulating miR-140/RAP1B

ICP4-Associated Activation of Rap1b Facilitates Herpes Simplex Virus Type I (HSV-1) Infection in Human Corneal Epithelial Cells