RANKL Polyclonal antibody

RANKL Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, Cell treatment, ELISA

Conjugate

Unconjugated

Cat no : 23408-1-AP

Synonyms

CD254, hRANKL2, ODF, OPGL, OPTB2, Osteoprotegerin ligand, RANKL, sOdf, TNFSF11, TRANCE



Tested Applications

Positive IHC detected inhuman osteosarcoma tissue, human colon tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 23408-1-AP is RANKL Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RANKL fusion protein Ag19975 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor (ligand) superfamily, member 11
Calculated molecular weight 317 aa, 35 kDa
GenBank accession numberBC074890
Gene symbol TNFSF11
Gene ID (NCBI) 8600
RRIDAB_2879273
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TNFSF11 also known as RANKL, is a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. RANKL is a polypeptide of 217 amino acids that exerts its biological activity both in a transmembrane form of about 40-45 kDa and in soluble one of 31 kDa (PMID: 15308315). The membrane-bound RANKL (mRANKL) is cleaved into a sRANKL by the metalloprotease-disintegrin TNF-alpha convertase (TACE) or a related metalloprotease (MP). RANKL induces osteoclast formation through its receptor, RANK, which transduces signals by recruiting adaptor molecules, such as the TNF receptor-associated factor (TRAF) family of proteins. RANKL was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. RANKL was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis.

Protocols

Product Specific Protocols
IHC protocol for RANKL antibody 23408-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Extracell Vesicles

Exosomes derived from osteogenic tumor activate osteoclast differentiation and concurrently inhibit osteogenesis by transferring COL1A1-targeting miRNA-92a-1-5p.

Authors - Lijuan Yu
mouse,humanWB

Aging Cell

Pyrroloquinoline quinone alleviates natural aging-related osteoporosis via a novel MCM3-Keap1-Nrf2 axis-mediated stress response and Fbn1 upregulation

Authors - Jie Li
ratIF

J Control Release

Sialic acid-modified chitosan oligosaccharide-based biphasic calcium phosphate promote synergetic bone formation in rheumatoid arthritis therapy.

Authors - Xiao-Ling Xu
mouseWB

Mol Metab

Loss of chemerin triggers bone remodeling in vivo and in vitro.

Authors - Long Han
mouseWB

Aging (Albany NY)

Increased expression of osteopontin in subchondral bone promotes bone turnover and remodeling, and accelerates the progression of OA in a mouse model.

Authors - Chuangxin Lin
mouseCell treatment

Int Immunopharmacol

Clearance of senescent cells by navitoclax (ABT263) rejuvenates UHMWPE-induced osteolysis

Authors - Weiping Su