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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated RALYL Monoclonal antibody

RALYL Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1F3F4

Cat no : CL594-67534

Synonyms

hnRNP core protein C like 3, HNRPCL3, hRALYL, RALY RNA binding protein like, RALYL, RNA binding Raly like protein

Validation Data Gallery

Applications:
  • IF Staining of HepG2 using CL594-67534

    IF Staining of HepG2 using CL594-67534

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 RALYL antibody (CL594-67534, Clone: 1F3F4 ) at dilution of 1:200.

  • IF Staining of HepG2 using CL594-67534

    IF Staining of HepG2 using CL594-67534

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 RALYL antibody (CL594-67534, Clone: 1F3F4 ) at dilution of 1:100.

  • FC experiment of HepG2 using CL594-67534

    FC experiment of HepG2 using CL594-67534

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human RALYL (CL594-67534, Clone:1F3F4) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67534-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL594-67534 targets RALYL in IF, FC (Intra) applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RALYL fusion protein Ag11676 相同性解析による交差性が予測される生物種
Full Name RALY RNA binding protein-like
Calculated molecular weight 291 aa, 32 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberBC031090
Gene symbol RALYL
Gene ID (NCBI) 138046
RRIDAB_2920153
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RALY RNA binding protein-like (RALYL) belongs to the RALY subfamily. RALYL locates in 8q21.2, the function of which is RNA-binding and nucleotide binding. The RALYL high expression in the normal adrenal, kidney and brain (Shyamsundar et al., 2005). Furthermore, RALYL expression down-regulated correlates with mental disorder (Lee et al., 2007), Parkinson's disease (Moran et al., 2006), brain cancer (Maris et al., 2008), adrenal cancer (Giordano et al., 2009) and kidney cancer (Higgins et al., 2003). The expression of RALYL was significantly low in Clear Cell Renal Cell Carcinoma and correlated with a poor prognosis in a large number of clinical samples. Our findings showed that RALYL may be a potential therapeutic target as well as a poor prognostic

Protocols

Product Specific Protocols
IF protocol for CL594 RALYL antibody CL594-67534Download protocol
Standard Protocols
Click here to view our Standard Protocols