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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated RALY Monoclonal antibody

RALY Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2B10C3

Cat no : CL594-68011

Synonyms

Autoantigen p542, hnRNP core protein C like 2, HNRPCL2, P542, RALY, RNA binding protein Raly

Validation Data Gallery

Applications:
  • IF Staining of A431 using CL594-68011

    IF Staining of A431 using CL594-68011

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite®594 RALY antibody (CL594-68011, Clone: 2B10C3 ) at dilution of 1:200.

  • FC experiment of A431 using CL594-68011

    FC experiment of A431 using CL594-68011

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human RALY (CL594-68011, Clone:2B10C3) (red), or 0.4 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive IF detected inA431 cells
Positive FC detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 68011-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL594-68011 targets RALY in IF, FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RALY fusion protein Ag12250 相同性解析による交差性が予測される生物種
Full Name RNA binding protein, autoantigenic (hnRNP-associated with lethal yellow homolog (mouse))
Calculated molecular weight 307 aa, 33 kDa
Observed molecular weight37 kDa
GenBank accession numberBC103753
Gene symbol RALY
Gene ID (NCBI) 22913
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RNA-binding protein RALY is a protein that in humans is encoded by the RALY gene. In infectious mononucleosis, anti-EBNA-1 antibodies are produced which cross-react with multiple normal human proteins. The cross-reactivity is due to anti-gly/ala antibodies that cross-react with host proteins containing configurations like those in the EBNA-1 repeat. One such antigen is RALY which is a member of the heterogeneous nuclear ribonucleoprotein gene family.

Protocols

Product Specific Protocols
IF protocol for CL594 RALY antibody CL594-68011Download protocol
FC protocol for CL594 RALY antibody CL594-68011Download protocol
Standard Protocols
Click here to view our Standard Protocols