• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated RALBP1 Monoclonal antibody

RALBP1 Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B2F5

Cat no : CL488-67359

Synonyms

76 kDa Ral interacting protein, DNP SG ATPase, Ral interacting protein 1, ralA binding protein 1, RALBP1, RIP1, RLIP1, RLIP76

Validation Data Gallery

Applications:
  • IF Staining of A431 using CL488-67359

    IF Staining of A431 using CL488-67359

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite® Plus 488 RALBP1 antibody (CL488-67359, Clone: 1B2F5 ) at dilution of 1:200.

  • FC experiment of HEK-293 using CL488-67359

    FC experiment of HEK-293 using CL488-67359

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RALBP1 (CL488-67359, Clone:1B2F5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inA431 cells
Positive FC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy RALBP1 Ready-To-Use IHC Kit. RALBP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67359-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-67359 targets RALBP1 in IF, FC (Intra) applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RALBP1 fusion protein Ag28618 相同性解析による交差性が予測される生物種
Full Name ralA binding protein 1
Calculated molecular weight 655 aa, 76 kDa
Observed molecular weight 95 kDa
GenBank accession numberBC013126
Gene symbol RALBP1
Gene ID (NCBI) 10928
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 RALBP1 antibody CL488-67359Download protocol
FC protocol for CL Plus 488 RALBP1 antibody CL488-67359Download protocol
Standard Protocols
Click here to view our Standard Protocols