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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RALBP1 Monoclonal antibody

RALBP1 Monoclonal Antibody for WB, FC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1B2F5

Cat no : 67359-1-Ig

Synonyms

76 kDa Ral interacting protein, DNP SG ATPase, Ral interacting protein 1, ralA binding protein 1, RALBP1, RIP1, RLIP1, RLIP76

Validation Data Gallery

Applications:
  • WB analysis of NIH/3T3 using 67359-1-Ig

    WB analysis of NIH/3T3 using 67359-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67359-1-Ig (RALBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 67359-1-Ig

    WB analysis of Jurkat using 67359-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67359-1-Ig (RALBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 67359-1-Ig

    WB analysis of HEK-293 using 67359-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67359-1-Ig (RALBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of LNCaP using 67359-1-Ig

    WB analysis of LNCaP using 67359-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67359-1-Ig (RALBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 67359-1-Ig

    WB analysis of MCF-7 using 67359-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67359-1-Ig (RALBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • FC experiment of HEK-293 using 67359-1-Ig

    FC experiment of HEK-293 using 67359-1-Ig

    1X10^6 HEK-293 cells were stained with 0.20 ug/test Anti-Human RALBP1 (67359-1-Ig, Clone:1B2F5) (red) or 0.20 ug control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000. Fixed with90% MeOH.

Tested Applications

Positive WB detected inNIH/3T3 cells, Jurkat cells, HEK-293 cells, LNCaP cells, MCF-7 cells
Positive FC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy RALBP1 Ready-To-Use IHC Kit. RALBP1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

67359-1-Ig targets RALBP1 in WB, FC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RALBP1 fusion protein Ag28618 相同性解析による交差性が予測される生物種
Full Name ralA binding protein 1
Calculated molecular weight 655 aa, 76 kDa
Observed molecular weight 95 kDa
GenBank accession numberBC013126
Gene symbol RALBP1
Gene ID (NCBI) 10928
RRIDAB_2882611
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RALBP1 antibody 67359-1-IgDownload protocol
FC protocol for RALBP1 antibody 67359-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols