RAD51AP1 Monoclonal antibody
RAD51AP1 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
2D8B1
Cat no : 68560-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, SW480 cells, NCCIT cells, Daudi cells, HaCaT cells, Jurkat cells, K-562 cells, NIH/3T3 cells, 4T1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Sample-dependent, check data in validation data gallery | |
Product Information
68560-1-Ig targets RAD51AP1 in WB, ELISA applications and shows reactivity with Human, mouse samples.
| Tested Reactivity | Human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | RAD51AP1 fusion protein Ag28385 相同性解析による交差性が予測される生物種 |
| Full Name | RAD51 associated protein 1 |
| Calculated molecular weight | 38 kDa |
| Observed molecular weight | 38-40 kDa |
| GenBank accession number | BC016330 |
| Gene symbol | RAD51AP1 |
| Gene ID (NCBI) | 10635 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Homologous recombination (HR) is an important pathway for the elimination of DNA double-strand breaks (DSBs) and other deleterious lesions from chromosomes. The eukaryotic HR reaction is mediated by RAD51 [PMID:12456786]. RAD51AP1 (RAD51 associated protein 1), so named because it can interact with human RAD51, is a vertebrate-specific protein that enhances the ability of RAD51 to mediate the D-loop reaction. RAD51AP1 knockdown by siRNA leads to cellular sensitivity to DNA damaging agents and elevated DNA damage-induced chromatid breaks. Enhancement of the RAD51 recombinase activity by RAD51AP1 is contingent upon the physical interaction of the two HR factors [PMID:17996710,17996711].
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RAD51AP1 antibody 68560-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
