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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RAD23B Polyclonal antibody

RAD23B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12121-1-AP

Synonyms

HHR23B, HR23B, P58, RAD23B

Validation Data Gallery

Applications:
  • WB analysis of Jurkat using 12121-1-AP

    WB analysis of Jurkat using 12121-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12121-1-AP (RAD23B antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse kidney using 12121-1-AP

    WB analysis of mouse kidney using 12121-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12121-1-AP (RAD23B antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • WB analysis of A431 using 12121-1-AP

    WB analysis of A431 using 12121-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 12121-1-AP (RAD23B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 12121-1-AP

    WB analysis of HEK-293 using 12121-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12121-1-AP (RAD23B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 12121-1-AP

    WB analysis of Jurkat using 12121-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12121-1-AP (RAD23B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 12121-1-AP

    WB analysis of HEK-293 using 12121-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12121-1-AP (RAD23B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • WB analysis of A431 using 12121-1-AP

    WB analysis of A431 using 12121-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 12121-1-AP (RAD23B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 12121-1-AP

    IHC staining of human breast cancer using 12121-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12121-1-AP (RAD23B antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAD23B antibody included
  • IHC staining of human breast cancer using 12121-1-AP

    IHC staining of human breast cancer using 12121-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12121-1-AP (RAD23B antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAD23B antibody included
  • IHC staining of human liver using 12121-1-AP

    IHC staining of human liver using 12121-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 12121-1-AP (RAD23B antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with RAD23B antibody included
  • IHC staining of human liver using 12121-1-AP

    IHC staining of human liver using 12121-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 12121-1-AP (RAD23B antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with RAD23B antibody included
  • IHC staining of human breast cancer using 12121-1-AP

    IHC staining of human breast cancer using 12121-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 12121-1-AP (RAD23B antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with RAD23B antibody included
  • IHC staining of human breast cancer using 12121-1-AP

    IHC staining of human breast cancer using 12121-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 12121-1-AP (RAD23B antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with RAD23B antibody included

Tested Applications

Positive WB detected inJurkat cells, A431 cells, HEK-293 cells, mouse kidney tissue
Positive IHC detected inhuman breast cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy RAD23B Ready-To-Use IHC Kit. RAD23B primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12121-1-AP targets RAD23B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAD23B fusion protein Ag2763 相同性解析による交差性が予測される生物種
Full Name RAD23 homolog B (S. cerevisiae)
Calculated molecular weight 409 aa, 43 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC020973
Gene symbol RAD23B
Gene ID (NCBI) 5887
RRIDAB_10641048
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RAD23B, also named as p58, hHR23B and HR23B, belongs to the RAD23 family. It plays a central role both in proteosomal degradation of misfolded proteins and DNA repair. RAD23B is a central component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulun that are retrotranslocated in the cytosol. Involved in DNA excision repair by stabilizing XPC protein. It may play a part in DNA damage recognition and/or in altering chromatin structure to allow access by damage-processing enzymes. RAD23B has two subunit: Heterodimer of a 125 kDa subunit (p125) and of a 58 kDa subunit (p58).

Protocols

Product Specific Protocols
WB protocol for RAD23B antibody 12121-1-APDownload protocol
IHC protocol for RAD23B antibody 12121-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Immunol

PDLIM7 Synergizes With PDLIM2 and p62/Sqstm1 to Inhibit Inflammatory Signaling by Promoting Degradation of the p65 Subunit of NF-κB.

Authors - Aya Jodo
View Article
pigWB

Mol Cell Proteomics

The mechano-ubiquitinome of articular cartilage: Differential ubiquitination and activation of a group of ER-associated DUBs and ER stress regulators

Authors - Nitchakarn Kaokhum
View Article
humanWB,IHC

Int J Biol Sci

Deubiquitinase PSMD7 promotes the proliferation, invasion, and cisplatin resistance of gastric cancer cells by stabilizing RAD23B.

Authors - Jianjiang Wang
View Article
humanWB

Front Oncol

PMID: 31249811

Authors - Ma-Sha Huang
View Article
ratWB

PLoS One

Proteins with Intrinsically Disordered Domains Are Preferentially Recruited to Polyglutamine Aggregates.

Authors - Maggie P Wear
View Article
humanWB

J Cancer

PMID: 31772670

Authors - Li-Ming Tan
View Article