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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated RACGAP1 Monoclonal antibody

RACGAP1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

6E10A2

Cat no : CL488-66056

Synonyms

HsCYK 4, ID GAP, KIAA1478, Male germ cell RacGap, MgcRacGAP, RACGAP1

Validation Data Gallery

Applications:
  • IF Staining of HeLa using CL488-66056

    IF Staining of HeLa using CL488-66056

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CL488-66056 (RACGAP1 antibody) at dilution of 1:100.

Tested Applications

Positive IF detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66056-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL488-66056 is RACGAP1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RACGAP1 fusion protein Ag6944 相同性解析による交差性が予測される生物種
Full Name Rac GTPase activating protein 1
Calculated molecular weight 632 aa, 71 kDa
Observed molecular weight 71 kDa
GenBank accession numberBC032754
Gene symbol RACGAP1
Gene ID (NCBI) 29127
RRIDAB_2883243
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Rac GTPase-activating protein 1 (RACGAP1), localized in the mitotic spindle, has guanosine triphosphatase-activating protein (GAP) activity for the Rho family GTPases. RACGAP1 has an important influence on the initiation of cytokinesis, control of cell growth and differentiation, regulation of spermatogenesis, and tumor metastasis.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 RACGAP1 antibody CL488-66056Download protocol
Standard Protocols
Click here to view our Standard Protocols