• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RACGAP1 Polyclonal antibody

RACGAP1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30042-1-AP

Synonyms

HsCYK 4, ID GAP, KIAA1478, Male germ cell RacGap, MgcRacGAP, RACGAP1

Validation Data Gallery

Applications:
  • WB analysis using 30042-1-AP

    WB analysis using 30042-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30042-1-AP (RACGAP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 30042-1-AP

    IHC staining of mouse testis using 30042-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30042-1-AP (RACGAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 30042-1-AP

    IHC staining of mouse testis using 30042-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30042-1-AP (RACGAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of MCF-7 using 30042-1-AP

    IF Staining of MCF-7 using 30042-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using RACGAP1 antibody (30042-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, K-562 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

30042-1-AP targets RACGAP1 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RACGAP1 fusion protein Ag32468 相同性解析による交差性が予測される生物種
Full Name Rac GTPase activating protein 1
Calculated molecular weight 632 aa, 71 kDa
Observed molecular weight71 kDa
GenBank accession numberBC032754
Gene symbol RACGAP1
Gene ID (NCBI) 29127
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RACGAP1 antibody 30042-1-APDownload protocol
IHC protocol for RACGAP1 antibody 30042-1-APDownload protocol
IF protocol for RACGAP1 antibody 30042-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols