RACGAP1 Polyclonal antibody

Name: RACGAP1 Polyclonal antibody
Brand: Proteintech
SKU: 13739-1-AP

RACGAP1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 13739-1-AP

Synonyms

HsCYK 4, ID GAP, KIAA1478, Male germ cell RacGap, MgcRacGAP, RACGAP1

Validation Data Gallery

Applications:

AllWBIP

WB analysis of K-562 using 13739-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 13739-1-AP (RACGAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 13739-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 13739-1-AP (RACGAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 13739-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 13739-1-AP (RACGAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of RAW 264.7 using 13739-1-AP
RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 13739-1-AP (RACGAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of mouse testis using 13739-1-AP
IP result of anti-RACGAP1 (IP:13739-1-AP, 4ug; Detection:13739-1-AP 1:500) with mouse testis tissue lysate 4000ug.
IP experiment of HeLa cells using 13739-1-AP
IP result of anti-RACGAP1 (13739-1-AP for IP and Detection).

-/ -

Cat No. 13739-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	K-562 cells, HeLa cells, RAW 264.7 cells, mouse testis tissue
Positive IP detected in	mouse testis tissue, HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Sample-dependent, check data in validation data gallery

Published Applications

WB	See 7 publications below
IHC	See 6 publications below
IF	See 2 publications below

Product Information

13739-1-AP targets RACGAP1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RACGAP1 fusion protein Ag4676 相同性解析による交差性が予測される生物種
Full Name	Rac GTPase activating protein 1
Calculated molecular weight	632 aa, 71 kDa
Observed molecular weight	71 kDa
GenBank accession number	BC032754
Gene symbol	RACGAP1
Gene ID (NCBI)	29127
RRID	AB_2176276
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Rac GTPase-activating protein 1 (RACGAP1), localized in the mitotic spindle, has guanosine triphosphatase-activating protein (GAP) activity for the Rho family GTPases. RACGAP1 has an important influence on the initiation of cytokinesis, control of cell growth and differentiation, regulation of spermatogenesis, and tumor metastasis.

Protocols

Product Specific Protocols
WB protocol for RACGAP1 antibody 13739-1-AP	Download protocol
IP protocol for RACGAP1 antibody 13739-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC	Front Cell Dev Biol PRC1 and RACGAP1 are Diagnostic Biomarkers of Early HCC and PRC1 Drives Self-Renewal of Liver Cancer Stem Cells. Authors - Shixin Liao View Article
mouse	WB,IHC	Front Pharmacol Effects of Shenkang Pills on Early-Stage Diabetic Nephropathy in db/db Mice via Inhibiting AURKB/RacGAP1/RhoA Signaling Pathway. Authors - Fujing Wang View Article
mouse	IF	Mol Biol Cell Chromokinesin Kif4 promotes proper anaphase in mouse oocyte meiosis. Authors - Carissa M Heath View Article
human	WB,IHC	Cancers (Basel) Lambda-Carrageenan Enhances the Effects of Radiation Therapy in Cancer Treatment by Suppressing Cancer Cell Invasion and Metastasis through Racgap1 Inhibition. Authors - Ping-Hsiu Wu View Article
human	WB	Cell Death Dis Pseudogene RACGAP1P activates RACGAP1/Rho/ERK signalling axis as a competing endogenous RNA to promote hepatocellular carcinoma early recurrence. Authors - Meng-Yao Wang View Article
human	WB,IHC,IF	Cell Death Dis PLAGL2 promotes bladder cancer progression via RACGAP1/RhoA GTPase/YAP1 signaling Authors - Hualin Chen View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RACGAP1 Polyclonal antibody

RACGAP1 Polyclonal Antibody for WB, IP, ELISA

Validation Data Gallery

WB analysis of K-562 using 13739-1-AP

WB analysis of mouse testis using 13739-1-AP

WB analysis of HeLa using 13739-1-AP

WB analysis of RAW 264.7 using 13739-1-AP

IP experiment of mouse testis using 13739-1-AP

IP experiment of HeLa cells using 13739-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

PRC1 and RACGAP1 are Diagnostic Biomarkers of Early HCC and PRC1 Drives Self-Renewal of Liver Cancer Stem Cells.

Effects of Shenkang Pills on Early-Stage Diabetic Nephropathy in db/db Mice via Inhibiting AURKB/RacGAP1/RhoA Signaling Pathway.

Chromokinesin Kif4 promotes proper anaphase in mouse oocyte meiosis.

Lambda-Carrageenan Enhances the Effects of Radiation Therapy in Cancer Treatment by Suppressing Cancer Cell Invasion and Metastasis through Racgap1 Inhibition.

Pseudogene RACGAP1P activates RACGAP1/Rho/ERK signalling axis as a competing endogenous RNA to promote hepatocellular carcinoma early recurrence.

PLAGL2 promotes bladder cancer progression via RACGAP1/RhoA GTPase/YAP1 signaling