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  • Featured Product
  • KD/KO Validated

RABL3 Polyclonal antibody

RABL3 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 11916-1-AP

Synonyms

Rab like protein 3, RABL3

Validation Data Gallery

Applications:
  • WB analysis using 11916-1-AP

    WB analysis using 11916-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11916-1-AP (RABL3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • WB analysis of human lung using 11916-1-AP

    WB analysis of human lung using 11916-1-AP

    human lung tissue were subjected to SDS PAGE followed by western blot with 11916-1-AP (RABL3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of human brain using 11916-1-AP

    WB analysis of human brain using 11916-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11916-1-AP (RABL3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 11916-1-AP

    WB analysis of mouse brain using 11916-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 11916-1-AP (RABL3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of HUVEC using 11916-1-PBS

    WB analysis of HUVEC using 11916-1-PBS

    HUVEC cells were subjected to SDS PAGE followed by western blot with 11916-1-AP (RABL3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 11916-1-AP

    WB analysis of HEK-293 using 11916-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11916-1-AP (RABL3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse lung using 11916-1-AP

    IP experiment of mouse lung using 11916-1-AP

    IP result of anti-RABL3 (IP:11916-1-AP, 3ug; Detection:11916-1-AP 1:300) with mouse lung tissue lysate 4000ug.

  • IHC staining of human breast cancer using 11916-1-AP

    IHC staining of human breast cancer using 11916-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11916-1-AP (RABL3 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human breast cancer using 11916-1-AP

    IHC staining of human breast cancer using 11916-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11916-1-AP (RABL3 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human liver cancer using 11916-1-AP

    IHC staining of human liver cancer using 11916-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11916-1-AP (RABL3 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver cancer using 11916-1-AP

    IHC staining of human liver cancer using 11916-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11916-1-AP (RABL3 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human breast cancer using 11916-1-AP

    IHC staining of human breast cancer using 11916-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 11916-1-AP (RABL3 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human breast cancer using 11916-1-AP

    IHC staining of human breast cancer using 11916-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 11916-1-AP (RABL3 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human cervical cancer using 11916-1-AP

    IHC staining of human cervical cancer using 11916-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11916-1-AP (RABL3 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human cervical cancer using 11916-1-AP

    IHC staining of human cervical cancer using 11916-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11916-1-AP (RABL3 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human kidney using 11916-1-AP

    IHC staining of human kidney using 11916-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 11916-1-AP (RABL3 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 11916-1-AP

    IHC staining of human kidney using 11916-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 11916-1-AP (RABL3 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human kidney using 11916-1-AP

    IHC staining of human kidney using 11916-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 11916-1-AP (RABL3 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 11916-1-AP

    IHC staining of human kidney using 11916-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 11916-1-AP (RABL3 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of A549 using 11916-1-AP

    IF Staining of A549 using 11916-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using RABL3 antibody (11916-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • FC experiment of HEK-293 using 11916-1-AP

    FC experiment of HEK-293 using 11916-1-AP

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human RABL3 (11916-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHEK-293 cells, mouse brain tissue, human brain tissue, human lung tissue, HUVEC cells, U-251 cells
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue, human cervical cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells
Positive FC detected inHEK-293 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

11916-1-AP targets RABL3 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RABL3 fusion protein Ag2531 相同性解析による交差性が予測される生物種
Full Name RAB, member of RAS oncogene family-like 3
Calculated molecular weight 236 aa, 26 kDa
Observed molecular weight 26 kDa
GenBank accession numberBC020832
Gene symbol RABL3
Gene ID (NCBI) 285282
RRIDAB_2175960
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The gene of Rabl3 is located on 3q13.3 and is composed of eight exons and seven introns. It is a member of the Rab subfamily, the largest group in the Ras superfamily of small guanosine triphosphatases (GTPases).

Protocols

Product Specific Protocols
WB protocol for RABL3 antibody 11916-1-APDownload protocol
IHC protocol for RABL3 antibody 11916-1-APDownload protocol
IF protocol for RABL3 antibody 11916-1-APDownload protocol
IP protocol for RABL3 antibody 11916-1-APDownload protocol
FC protocol for RABL3 antibody 11916-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncol Rep

Evaluation of the novel gene Rabl3 in the regulation of proliferation and motility in human cancer cells.

Authors - Li Qi Q
  • KD Validated
View Article
humanWB

Med Sci Monit

High Expression of Rab-like 3 (Rabl3) is Associated with Poor Survival of Patients with Non-Small Cell Lung Cancer via Repression of MAPK8/9/10-Mediated Autophagy.

Authors - Weihua Zhang
  • KD Validated
View Article