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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RAB8A Polyclonal antibody

RAB8A Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, canine, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 30636-1-AP

Synonyms

MEL, Oncogene c mel, RAB8, RAB8A, Ras related protein Rab 8A

Validation Data Gallery

Applications:
  • WB analysis using 30636-1-AP

    WB analysis using 30636-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30636-1-AP (RAB8A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of MDCK using 30636-1-AP

    IF Staining of MDCK using 30636-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using RAB8A antibody (30636-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of NIH/3T3 using 30636-1-AP

    IF Staining of NIH/3T3 using 30636-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using RAB8A antibody (30636-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA431 cells, HeLa cells, HepG2 cells
Positive IF detected inNIH/3T3 cells, MDCK cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

30636-1-AP targets RAB8A in WB, IF, ELISA applications and shows reactivity with Human, canine, mouse samples.

Tested Reactivity Human, canine, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB8A fusion protein Ag30567 相同性解析による交差性が予測される生物種
Full Name RAB8A, member RAS oncogene family
Calculated molecular weight 24 kDa
Observed molecular weight 24 kDa
GenBank accession numberNM_005370
Gene symbol RAB8A
Gene ID (NCBI) 4218
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The Rab8 GTPase is a member of the Ras superfamily that functions in protein transport and membrane restructuring. RAB8 has two isoforms, RAB8A and RAB8B, that share 40% amino acid identity in the C-terminal variable region. RAB8 activity is regulated by specific guanine nucleotide exchange factors (GEFs), that mediate GTP loading, and GTPase activating proteins (GAPs) that convert them into the inactive GDP-bound form. RAB8 participates in recycling of the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors to the dendritic spine surface as well as in the intracellular transport of the metabotropic glutamate receptor type 1 in neuronal cells. Rab8 is activated at the base of the cilium by its guanine nucleotide exchanger Rabin8. The antibody 11792-1-AP can detect both RAB8A and RAB8B.

Protocols

Product Specific Protocols
WB protocol for RAB8A antibody 30636-1-APDownload protocol
IF protocol for RAB8A antibody 30636-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols