Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 11947-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11947-1-AP (RAB5A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis using 11947-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11947-1-AP (RAB5A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 11947-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 11947-1-AP (RAB5A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 11947-1-AP
IP result of anti-RAB5A (IP:11947-1-AP, 4ug; Detection:11947-1-AP 1:10000) with mouse brain tissue lysate 1680 ug.
IHC staining of human gliomas using 11947-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11947-1-AP (RAB5A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 11947-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11947-1-AP (RAB5A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 11947-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11947-1-AP (RAB5A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas using 11947-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11947-1-AP (RAB5A Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human pancreas using 11947-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11947-1-AP (RAB5A Antibody) at dilution of 1:200 (under 40x lens).

Featured Product
KD/KO Validated

RAB5A Polyclonal antibody

RAB5A Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 11947-1-AP

Publications(45)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

RAB5, RAB5A, Ras related protein Rab 5A

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (45)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse brain tissue, HeLa cells, human brain tissue, rat brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human gliomas tissue, mouse brain tissue, human pancreas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 8 publications below
WB	See 31 publications below
IHC	See 7 publications below
IF	See 20 publications below
IP	See 2 publications below

Product Information

11947-1-AP targets RAB5A in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, monkey, zebrafish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2549 Product name: Recombinant human RAB5A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-215 aa of BC001267 Sequence: MASRGATRPNGPNTGNKICQFKLVLLGESAVGKSSLVLRFVKGQFHEFQESTIGAAFLTQTVCLDDTTVKFEIWDTAGQERYHSLAPMYYRGAQAAIVVYDITNEESFARAKNWVKELQRQASPNIVIALSGNKADLANKRAVDFQEAQSYADDNSLLFMETSAKTSMNVNEIFMAIAKKLPKNEPQNPGANSARGRGVDLTEPTQPTRNQCCSN 相同性解析による交差性が予測される生物種
Full Name	RAB5A, member RAS oncogene family
Calculated molecular weight	215 aa, 24 kDa
Observed molecular weight	24 kDa
GenBank accession number	BC001267
Gene Symbol	RAB5A
Gene ID (NCBI)	5868
RRID	AB_2269388
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P20339
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IHC protocol for RAB5A antibody 11947-1-AP	Download protocol
IP protocol for RAB5A antibody 11947-1-AP	Download protocol
WB protocol for RAB5A antibody 11947-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Nat Genet Identification of TMEM230 mutations in familial Parkinson's disease. Authors - Han-Xiang Deng View Article
human	WB,IP	Autophagy Live imaging of intra-lysosome pH in cell lines and primary neuronal culture using a novel genetically encoded biosensor. Authors - Amy H Ponsford View Article
mouse	WB	Autophagy RAB7 activity is required for the regulation of mitophagy in oocyte meiosis and oocyte quality control during ovarian aging. Authors - Xin Jin View Article
mouse	WB,IHC	Diabetes Atorvastatin Targets the Islet Mevalonate Pathway to Dysregulate mTOR Signaling and Reduce β-Cell Functional Mass. Authors - Linyan Shen KD Validated View Article
rat	WB, IF	Sci Signal Semaphorin 3A activates the guanosine triphosphatase Rab5 to promote growth cone collapse and organize callosal axon projections. Authors - Kong-Yan Wu View Article
human	WB,IF,IP	Elife Capping protein regulates endosomal trafficking by controlling F-actin density around endocytic vesicles and recruiting RAB5 effectors. Authors - Dawei Wang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 11947-1-AP

WB analysis using 11947-1-AP

WB analysis of human brain using 11947-1-AP

IP experiment of mouse brain using 11947-1-AP

IHC staining of human gliomas using 11947-1-AP

IHC staining of human gliomas using 11947-1-AP

IHC staining of mouse brain using 11947-1-AP

IHC staining of human pancreas using 11947-1-AP

IHC staining of human pancreas using 11947-1-AP

RAB5A Polyclonal antibody

RAB5A Polyclonal Antibody for WB, IP, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Identification of TMEM230 mutations in familial Parkinson's disease.

Live imaging of intra-lysosome pH in cell lines and primary neuronal culture using a novel genetically encoded biosensor.

RAB7 activity is required for the regulation of mitophagy in oocyte meiosis and oocyte quality control during ovarian aging.

Atorvastatin Targets the Islet Mevalonate Pathway to Dysregulate mTOR Signaling and Reduce β-Cell Functional Mass.

Semaphorin 3A activates the guanosine triphosphatase Rab5 to promote growth cone collapse and organize callosal axon projections.

Capping protein regulates endosomal trafficking by controlling F-actin density around endocytic vesicles and recruiting RAB5 effectors.