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  • KD/KO Validated

RAB3GAP1 Polyclonal antibody

RAB3GAP1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 21663-1-AP

Synonyms

KIAA0066, P130, Rab3 GAP, Rab3 GAP p130, RAB3 GTPase-activating protein 1, RAB3GAP, RAB3GAP1, RAB3GAP130, WARBM1



Tested Applications

Positive WB detected inHEK-293 cells, HL-60 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

21663-1-AP targets RAB3GAP1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat, mouse, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB3GAP1 fusion protein Ag16426 相同性解析による交差性が予測される生物種
Full Name RAB3 GTPase activating protein subunit 1 (catalytic)
Calculated molecular weight 981 aa, 111 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC146809
Gene symbol RAB3GAP1
Gene ID (NCBI) 22930
RRIDAB_10863124
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RAB3GAP1 (RAB3 GTPase-activating protein 1) is the catalytic subunit of the heterodimeric enzyme RAB3GAP (RAB3GTPase-activating protein), a key regulator of the RAB3 cycle, which controls calcium-mediated exocytosis of neurotransmitters and hormones. Mutations in RAB3GAP1 cause Warburg Micro syndrome (OMIM 600118) and Martsolf syndrome (OMIM 212720), phenotypically overlapping autosomal recessive conditions characterized by ocular and neurodevelopmental manifestations.

Protocols

Product Specific Protocols
WB protocol for RAB3GAP1 antibody 21663-1-APDownload protocol
IHC protocol for RAB3GAP1 antibody 21663-1-APDownload protocol
IF protocol for RAB3GAP1 antibody 21663-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
monkeyWB

J Cell Biol

Rab18 and a Rab18 GEF complex are required for normal ER structure.

Authors - Andreas Gerondopoulos
  • KD Validated
humanWB

EMBO J

COPI-TRAPPII activates Rab18 and regulates its lipid droplet association.

Authors - Chunman Li
human, mouseWB

BMC Biol

VAPB/ALS8 interacts with FFAT-like proteins including the p97 cofactor FAF1 and the ASNA1 ATPase.

Authors - Yorann Baron
mouseWB

J Neurosci

Mutant Huntingtin Impairs BDNF Release from Astrocytes by Disrupting Conversion of Rab3a-GTP into Rab3a-GDP.

Authors - Yan Hong
ratWB,IF

Toxicol Lett

Manganese-induced alpha-synuclein overexpression impairs synaptic vesicle fusion by disrupting the Rab3 cycle in primary cultured neurons.

Authors - Tong-Yu Wang
humanWB

Open Biol

Warburg Micro syndrome is caused by RAB18 deficiency or dysregulation.

Authors - Mark T Handley

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Sammy (Verified Customer) (11-05-2023)

Detects a band at the correct MW via western blotting. An unspecific band also detected around 40 kDa.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HUVEC
RAB3GAP1 Antibody Western Blot validation (1:1000 dilution) in HUVEC (Cat no:21663-1-AP)
FH

Bing (Verified Customer) (08-04-2021)

OK to probe RAB3GAP1, but sometimes produces an unspecific band nearby. 66688-1-Ig seems better.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: mouse brain lysate