Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of mouse lung using 12320-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 12320-1-AP (RAB3D Antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 12320-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 12320-1-AP (RAB3D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of SW 1990 using 12320-1-AP
SW 1990 cells were subjected to SDS PAGE followed by western blot with 12320-1-AP (RAB3D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse spleen using 12320-1-AP
mouse spleen tissue were subjected to SDS PAGE followed by western blot with 12320-1-AP (RAB3D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human stomach using 12320-1-AP
human stomach tissue were subjected to SDS PAGE followed by western blot with 12320-1-AP (RAB3D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human cerebellum using 12320-1-AP
human cerebellum tissue were subjected to SDS PAGE followed by western blot with 12320-1-AP (RAB3D antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 12320-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 12320-1-AP (RAB3D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse stomach using 12320-1-AP
mouse stomach tissue were subjected to SDS PAGE followed by western blot with 12320-1-AP (RAB3D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of SW 1990 using 12320-1-AP
IP result of anti-RAB3D (IP:12320-1-AP, 3ug; Detection:12320-1-AP 1:600) with SW 1990 cells lysate 2400ug.
IHC staining of human prostate cancer using 12320-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12320-1-AP (RAB3D antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 12320-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12320-1-AP (RAB3D antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 12320-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12320-1-AP (RAB3D antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 12320-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12320-1-AP (RAB3D antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

RAB3D Polyclonal antibody

RAB3D Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 12320-1-AP

Publications(18)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IP, IF, IHC, ELISA

Synonyms

D2 2, GOV, RAB16, RAB3D, RAD3D, Ras related protein Rab 3D

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (18)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse lung tissue, A549 cells, human cerebellum tissue, human stomach tissue, mouse spleen tissue, mouse stomach tissue, SW 1990 cells
Positive IP detected in	SW 1990 cells
Positive IHC detected in	human prostate cancer tissue, human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2400
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:400-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 9 publications below
IHC	See 9 publications below
IF	See 4 publications below

Product Information

12320-1-AP targets RAB3D in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, goat, rat, mouse, pig, canine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RAB3D fusion protein Ag2973 相同性解析による交差性が予測される生物種
Full Name	RAB3D, member RAS oncogene family
Calculated molecular weight	219 aa, 24 kDa
Observed molecular weight	28 kDa
GenBank accession number	BC016471
Gene Symbol	RAB3D
Gene ID (NCBI)	9545
RRID	AB_2177228
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O95716
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Rab3D is a member of the Rab3 subfamily, which comprises four isoforms (Rab3A, Rab3B, Rab3C, and Rab3D). Rab3 proteins typically associate with secretory vesicles, including synaptic vesicles, and have been implicated in the control of regulated exocytosis. Rab3D is widely expressed in adipocytes, exocrine glands and several haematopoietic cells, where it localizes to secretory granules and vesicles.

Protocols

Product Specific Protocols
WB protocol for RAB3D antibody 12320-1-AP	Download protocol
IHC protocol for RAB3D antibody 12320-1-AP	Download protocol
IP protocol for RAB3D antibody 12320-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
pig	WB	Mol Metab Maternal hyperglycemia induces alterations in hepatic amino acid, glucose and lipid metabolism of neonatal offspring: Multi-omics insights from a diabetic pig model Authors - Bachuki Shashikadze View Article
		Sci Signal RhoA inhibits neural differentiation in murine stem cells through multiple mechanisms. Authors - Junning Yang View Article
human	IHC	Mol Cancer Secretory RAB GTPase 3C modulates IL6-STAT3 pathway to promote colon cancer metastasis and is associated with poor prognosis. Authors - Yu-Chan Chang View Article
human	IHC	PLoS One Accumulation of secretory vesicles in the lacrimal gland epithelia is related to non-Sjögren's type dry eye in visual display terminal users. Authors - Mizuka Kamoi View Article
mouse	IF	Am J Physiol Gastrointest Liver Physiol Establishment of novel in vitro mouse chief cell and SPEM cultures identifies MAL2 as a marker of metaplasia in the stomach. Authors - Victoria G Weis View Article
human	WB	Cancer Manag Res The lncRNA HOXA11-AS regulates Rab3D expression by sponging miR-125a-5p promoting metastasis of osteosarcoma. Authors - Kun Cao View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse lung using 12320-1-AP

WB analysis of mouse lung using 12320-1-AP

WB analysis of SW 1990 using 12320-1-AP

WB analysis of mouse spleen using 12320-1-AP

WB analysis of human stomach using 12320-1-AP

WB analysis of human cerebellum using 12320-1-AP

WB analysis of A549 using 12320-1-AP

WB analysis of mouse stomach using 12320-1-AP

IP experiment of SW 1990 using 12320-1-AP

IHC staining of human prostate cancer using 12320-1-AP

IHC staining of human prostate cancer using 12320-1-AP

IHC staining of human breast cancer using 12320-1-AP

IHC staining of human breast cancer using 12320-1-AP

RAB3D Polyclonal antibody

RAB3D Polyclonal Antibody for WB, IP, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Maternal hyperglycemia induces alterations in hepatic amino acid, glucose and lipid metabolism of neonatal offspring: Multi-omics insights from a diabetic pig model

RhoA inhibits neural differentiation in murine stem cells through multiple mechanisms.

Secretory RAB GTPase 3C modulates IL6-STAT3 pathway to promote colon cancer metastasis and is associated with poor prognosis.

Accumulation of secretory vesicles in the lacrimal gland epithelia is related to non-Sjögren's type dry eye in visual display terminal users.

Establishment of novel in vitro mouse chief cell and SPEM cultures identifies MAL2 as a marker of metaplasia in the stomach.

The lncRNA HOXA11-AS regulates Rab3D expression by sponging miR-125a-5p promoting metastasis of osteosarcoma.