Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, human brain tissue, mouse lung tissue, rat brain tissues |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 2 publications below |
| IF | See 2 publications below |
Product Information
10788-1-AP targets RAB3C in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RAB3C fusion protein Ag1241 相同性解析による交差性が予測される生物種 |
| Full Name | RAB3C, member RAS oncogene family |
| Calculated molecular weight | 26 kDa |
| Observed molecular weight | 28 kDa |
| GenBank accession number | BC013033 |
| Gene Symbol | RAB3C |
| Gene ID (NCBI) | 115827 |
| RRID | AB_2177217 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96E17 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Rab proteins are small molecular weight GTPases that control vesicular traffic in eukaryotic cells. A subset of Rab proteins, the Rab3 proteins (Rab3A, Rab3B, Rab3C, and Rab3D) are thought to play an important role in regulated exocytosis of vesicles. Although Rab3 proteins are highly homologous, displaying 77-85% amino acid identity with greatest variance evident within the N- and C-terminal regions, their subcellular targets and functional roles remain somewhat distinct between them. RT-PCR analysis indicated that human Rab3C was expressed in the human brain, placenta, and lung.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RAB3C antibody 10788-1-AP | Download protocol |
| IHC protocol for RAB3C antibody 10788-1-AP | Download protocol |
| IP protocol for RAB3C antibody 10788-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A The GTPase Rab3b/3c-positive recycling vesicles are involved in cross-presentation in dendritic cells. | ||
PLoS Genet Cell fate specification in the lingual epithelium is controlled by antagonistic activities of Sonic hedgehog and retinoic acid. | ||
Front Endocrinol (Lausanne) Clinical identification of expressed proteins in adrenal medullary hyperplasia detected with hypertension | ||
Signal Transduct Target Ther Integrative analysis of DNA methylation and gene expression identified cervical cancer-specific diagnostic biomarkers. | ||
Signal Transduct Target Ther Integrative analysis of DNA methylation and gene expression identified cervical cancer-specific diagnostic biomarkers |