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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RAB38 Polyclonal antibody

RAB38 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12234-1-AP

Synonyms

Melanoma antigen NY MEL 1, NY MEL 1, RAB38, Ras related protein Rab 38, rrGTPbp

Validation Data Gallery

Applications:
  • WB analysis of NIH/3T3 using 12234-1-AP

    WB analysis of NIH/3T3 using 12234-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12234-1-AP (RAB38 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of A375 using 12234-1-AP

    WB analysis of A375 using 12234-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 12234-1-AP (RAB38 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 12234-1-AP

    WB analysis of NIH/3T3 using 12234-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12234-1-AP (RAB38 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 12234-1-AP

    IHC staining of human gliomas using 12234-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12234-1-AP (RAB38 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human gliomas using 12234-1-AP

    IHC staining of human gliomas using 12234-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12234-1-AP (RAB38 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung using 12234-1-AP

    IHC staining of human lung using 12234-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 12234-1-AP (RAB38 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung using 12234-1-AP

    IHC staining of human lung using 12234-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 12234-1-AP (RAB38 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A549 using 12234-1-AP

    IF Staining of A549 using 12234-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 12234-1-AP (RAB38 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inNIH/3T3 cells, A375 cells
Positive IHC detected inhuman gliomas tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12234-1-AP targets RAB38 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB38 fusion protein Ag2874 相同性解析による交差性が予測される生物種
Full Name RAB38, member RAS oncogene family
Calculated molecular weight 211 aa, 24 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC015808
Gene symbol RAB38
Gene ID (NCBI) 23682
RRIDAB_2877839
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RAB38 antibody 12234-1-APDownload protocol
IHC protocol for RAB38 antibody 12234-1-APDownload protocol
IF protocol for RAB38 antibody 12234-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EMBO Rep

Rab22A recruits BLOC-1 and BLOC-2 to promote the biogenesis of recycling endosomes.

Authors - Saurabh Shakya
View Article
mouseIF

JBMR Plus

Interspecies Single-Cell RNA-Seq Analysis Reveals the Novel Trajectory of Osteoclast Differentiation and Therapeutic Targets

Authors - Yasunori Omata
View Article