Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, human brain tissue, K-562 cells |
| Positive IHC detected in | human colon tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 6 publications below |
| WB | See 10 publications below |
| IHC | See 4 publications below |
| IF | See 2 publications below |
| CoIP | See 2 publications below |
Product Information
16182-1-AP targets RAB31-specific in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | RAB31, member RAS oncogene family |
| Calculated molecular weight | 22 kDa |
| Observed molecular weight | 22 kDa |
| GenBank accession number | NM_006868 |
| Gene Symbol | RAB31 |
| Gene ID (NCBI) | 11031 |
| RRID | AB_1851568 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13636 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RAB 31, also named as RAB22b, belongs to small GTP-binding proteins of the RAB family. Rab31 localizes in the trans-Golgi network (TGN) and endosomes. It is required for transport of mannose 6-phosphate receptors (MPRs) from the trans-Golgi network (TGN) to endosomes. This antibody can bind RAB31 specifically, and not react RAB22a.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for RAB31-specific antibody 16182-1-AP | Download protocol |
| WB protocol for RAB31-specific antibody 16182-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Biol Sci Rab31 promotes the invasion and metastasis of cervical cancer cells by inhibiting MAPK6 degradation.
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Front Oncol RAB31 Targeted by MiR-30c-2-3p Regulates the GLI1 Signaling Pathway, Affecting Gastric Cancer Cell Proliferation and Apoptosis.
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PLoS One Human liver cell trafficking mutants: characterization and whole exome sequencing. | ||
Int J Biochem Cell Biol Rab31 promotes activation of hepatic stellate cells by accelerating TGF-β receptor II complex endocytosis.
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Life Sci Upregulation of Rab31 is associated with poor prognosis and promotes colorectal carcinoma proliferation via the mTOR/p70S6K/Cyclin D1 signalling pathway.
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