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  • Featured Product
  • KD/KO Validated

RAB27A Polyclonal antibody

RAB27A Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (4)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17817-1-AP

Synonyms

GS2, GTP binding protein Ram, HsT18676, Rab 27, RAB27, RAB27A, RAM, Ras related protein Rab 27A

Validation Data Gallery

Applications:
  • WB analysis using 17817-1-AP

    WB analysis using 17817-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17817-1-AP (RAB27A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of K562 using 17817-1-AP

    WB analysis of K562 using 17817-1-AP

    WB result of RAB27A antibody (17817-1-AP, 1:3000) with si-Control and si-RAB7B transfected K562 cells.

  • WB analysis of Apoptosised HeLa using 17817-1-AP

    WB analysis of Apoptosised HeLa using 17817-1-AP

    Apoptosised HeLa cells were subjected to SDS PAGE followed by western blot with 17817-1-AP (RAB27A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of L02 using 17817-1-AP

    WB analysis of L02 using 17817-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 17817-1-AP (RAB27A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of K-562 using 17817-1-AP

    WB analysis of K-562 using 17817-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 17817-1-AP (RAB27A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 17817-1-AP

    WB analysis of HeLa using 17817-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17817-1-AP (RAB27A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of human liver using 17817-1-AP

    WB analysis of human liver using 17817-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 17817-1-AP (RAB27A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of K-562 using 17817-1-AP

    IP experiment of K-562 using 17817-1-AP

    IP result of anti-RAB27A (IP:17817-1-AP, 4ug; Detection:17817-1-AP 1:1000) with K-562 cells lysate 2760 ug.

  • IHC staining of human malignant melanoma using 17817-1-AP

    IHC staining of human malignant melanoma using 17817-1-AP

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 17817-1-AP (RAB27A antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with RAB27A antibody included
  • IHC staining of human malignant melanoma using 17817-1-AP

    IHC staining of human malignant melanoma using 17817-1-AP

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 17817-1-AP (RAB27A antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with RAB27A antibody included
  • IHC staining of human stomach using 17817-1-AP

    IHC staining of human stomach using 17817-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 17817-1-AP (RAB27A Antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with RAB27A antibody included
  • IHC staining of human stomach using 17817-1-AP

    IHC staining of human stomach using 17817-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 17817-1-AP (RAB27A Antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with RAB27A antibody included

Tested Applications

Positive WB detected inHL-60 cells, Apoptosised HeLa cells, human liver tissue, K562, K-562 cells, L02 cells, Jurkat cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman malignant melanoma tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy RAB27A Ready-To-Use IHC Kit. RAB27A primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

17817-1-AP targets RAB27A in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, Chicken, rat, pig, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB27A fusion protein Ag12136 相同性解析による交差性が予測される生物種
Full Name RAB27A, member RAS oncogene family
Calculated molecular weight 221 aa, 25 kDa
Observed molecular weight 25-27 kDa
GenBank accession numberBC107680
Gene symbol RAB27A
Gene ID (NCBI) 5873
RRIDAB_2176728
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Rab27A is a member of a large family of Ras-related small GTPases. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Recent studies suggest that Rab27A is associated with melanosomes in pigmented cells and regulates melanosome transport via its interaction with actin-based cellular motors such as myosin Va and myosin VIIa. RAB27A is also required for regulated secretion in cytotoxic T lymphocytes. Rab27A is implicated in several diseases, including Griscelli syndrome, choroideremia, and the Hermansky-Pudlak syndrome mouse model, gunmetal.

Protocols

Product Specific Protocols
WB protocol for RAB27A antibody 17817-1-APDownload protocol
IHC protocol for RAB27A antibody 17817-1-APDownload protocol
IP protocol for RAB27A antibody 17817-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Immunol

Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity.

Authors - Li Jianhua J
View Article
humanWB

Adv Sci (Weinh)

Exosome Release Is Regulated by mTORC1.

Authors - Wenchong Zou
View Article
humanWB

J Allergy Clin Immunol

A RAB27A 5' untranslated region structural variant associated with late-onset hemophagocytic lymphohistiocytosis and normal pigmentation.

Authors - Bianca Tesi
View Article
humanWB

Genes Dev

Cdx2 regulates endo-lysosomal function and epithelial cell polarity.

Authors - Gao Nan N
View Article
humanWB

Nat Commun

Exosomes maintain cellular homeostasis by excreting harmful DNA from cells.

Authors - Akiko Takahashi
  • KD Validated
View Article

Blood

Extracellular vesicles released by CD40/IL-4 stimulated chronic lymphocytic leukemia cells confer altered functional properties to CD4+ T cells.

Authors - Dawn T Smallwood
View Article