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  • KD/KO Validated

Rab23 Polyclonal antibody

Rab23 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11101-1-AP

Synonyms

HSPC137, RAB23, Ras related protein Rab 23



Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue, HEK-293 cells, human brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11101-1-AP targets Rab23 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Rab23 fusion protein Ag1588 相同性解析による交差性が予測される生物種
Full Name RAB23, member RAS oncogene family
Calculated molecular weight 237 aa, 27 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC015021
Gene symbol RAB23
Gene ID (NCBI) 51715
RRIDAB_2173784
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Rab23 is a novel member of large Rab small GTPase family. It plays important roles during embryonic development. The brain-enriched Rab23 is the only Rab protein that is known to have a distinct function during central nervous system development, playing an essential role as a negative regulator of the Sonic Hedgehog (Shh) pathway. RAB23 has recently been identified in mesangial cells (MCs), and could serve as a biomarker that indicates the severity of FSGS ( focal segmental glomerulosclerosis). Mutations in RAB23 may result in ACPS2 ( acrocephalopolysyndactyly type 2).

Protocols

Product Specific Protocols
WB protocol for Rab23 antibody 11101-1-APDownload protocol
IHC protocol for Rab23 antibody 11101-1-APDownload protocol
IP protocol for Rab23 antibody 11101-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IF

Curr Biol

Planar Cell Polarity Effector Proteins Inturned and Fuzzy Form a Rab23 GEF Complex.

Authors - Andreas Gerondopoulos
  • KD Validated
mouseWB,IHC

Elife

RAB23 coordinates early osteogenesis by repressing FGF10-pERK1/2 and GLI1.

Authors - Md Rakibul Hasan
humanWB,IF

Oncotarget

Rab23 promotes squamous cell carcinoma cell migration and invasion via integrin β1/Rac1 pathway.

Authors - Qiang Jian
  • KD Validated
humanWB

J Cell Sci

Differential role of Rab proteins in ciliary trafficking: Rab23 regulates smoothened levels.

Authors - Boehlke Christopher C
  • KD Validated
humanWB,IHC

J Cancer Res Clin Oncol

MicroRNA-367-3p directly targets RAB23 and inhibits proliferation, migration and invasion of bladder cancer cells and increases cisplatin sensitivity

Authors - Xifeng Wei
  • KD Validated
human,mouseWB,IF

Exp Dermatol

Silencing of Rab23 by siRNA inhibits ultraviolet B-induced melanogenesis via downregulation of PKA/CREB/MITF.

Authors - Xuanjin Wei
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Guorong (Verified Customer) (11-22-2022)

Multiple bands distributed at 50 and 75 kDa were observed . Does not represent the performance in other kind of samples

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: ARPE-19
Rab23 Antibody Western Blot validation (1:500 dilution) in ARPE-19 (Cat no:11101-1-AP)