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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647-conjugated Phospho-RIPK1 (Ser161) Monoclonal antibody

Phospho-RIPK1 (Ser161) Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1B2G1

Cat no : CL647-66854

Synonyms

Cell death protein RIP, FLJ39204, Receptor interacting protein 1, RIP, RIP 1, RIP1, RIPK1

Validation Data Gallery

Applications:
  • IF Staining of U2OS using CL647-66854

    IF Staining of U2OS using CL647-66854

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CoraLite® Plus 647 Phospho-RIPK1 (Ser161) antibody (CL647-66854, Clone: 1B2G1 ) at dilution of 1:400.

  • FC experiment of HEK-293T using CL647-66854

    FC experiment of HEK-293T using CL647-66854

    1X10^6 HEK-293T cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.13 ug CoraLite® Plus 647 Anti-Human Phospho-RIPK1 (Ser161) (CL647-66854, Clone:1B2G1) (red), or 0.13 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

  • FC experiment of HEK-293T using CL647-66854

    FC experiment of HEK-293T using CL647-66854

    1X10^6 HEK-293T cells were intracellularly stained with 0.13 ug CoraLite® Plus 647 Anti-Human Phospho-RIPK1 (Ser161) (CL647-66854, Clone:1B2G1) (red), or 0.13 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Tested Applications

Positive IF detected inU2OS cells
Positive FC detected inCalyculin A treated HEK-293T cells, HEK-293T cells
For other applications, we recommend the unconjugated version of this antibody, 66854-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.13 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL647-66854 targets Phospho-RIPK1 (Ser161) in IF, FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name receptor (TNFRSF)-interacting serine-threonine kinase 1
Calculated molecular weight 76 kDa
Observed molecular weight 74 kDa, 45 kDa
GenBank accession numberNM_003804
Gene symbol RIPK1
Gene ID (NCBI) 8737
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RIPK1(Receptor-interacting serine/threonine-protein kinase 1) is primarily involved in mediating TNF-R1-induced cell activation, apoptosis and necroptosis and belongs to a novel class of kinases thatfunction in cell survival and cell death mechanisms(PMID:22685397 ).It has 2 isoforms produced by alternative splicing.It also can exist as the protein with lower molecular weight about 25kd and 45kd in HAEC and HUVEC(PMID:22685397).

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 Phospho-RIPK1 (Ser161) antibody CL647-66854Download protocol
Standard Protocols
Click here to view our Standard Protocols