Phospho-eNOS (Thr495) Polyclonal antibody
Phospho-eNOS (Thr495) Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human and More (2)
Applications
WB, ELISA
Conjugate
Unconjugated
Cat no : 28939-1-AP
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | VEGF treated HUVEC cells, EGF treated HUVEC cells, Cobalt Chloride treated HUVEC cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 4 publications below |
Product Information
28939-1-AP targets Phospho-eNOS (Thr495) in WB, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | nitric oxide synthase 3 (endothelial cell) |
| Calculated molecular weight | 133 kDa |
| Observed molecular weight | 133 kDa |
| GenBank accession number | BC063294 |
| Gene symbol | NOS3 |
| Gene ID (NCBI) | 4846 |
| RRID | AB_2881234 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
Endothelial NOS (eNOS), also known as nitric oxide synthase 3 (NOS3), has a protective function in the cardiovascular system, which is attributed to NO production. Polymorphisms in NOS3 gene affects the susceptibility to several diseases such as hypertension, preeclampsia, diabetes mellitus, obesity, erectile dysfunction, and migraine.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-eNOS (Thr495) antibody 28939-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Ethnopharmacol Dahuang Zhechong pill attenuates hepatic sinusoidal capillarization in liver cirrhosis and hepatocellular carcinoma rat model via MK/integrin signaling pathway | ||
Drug Des Devel Ther Clopidogrel-Induced Gastric Injury in Rats is Attenuated by Stable Gastric Pentadecapeptide BPC 157. | ||
J Oncol Hax-1 Regulates Radiation-Induced Mitochondrial-Dependent Apoptosis of Uveal Melanoma Cells through PI3K/AKT/eNOS Pathway. | ||
Cytotherapy Porous gelatin microspheres implanted with adipose mesenchymal stromal cells promote angiogenesis via protein kinase B/endothelial nitric oxide synthase signaling pathway in bladder reconstruction |
