Phospho-Hamartin/TSC1 (Ser511) Recombinant antibody
Phospho-Hamartin/TSC1 (Ser511) Recombinant Antibody for WB, FC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse
Applications
WB, FC, ELISA
Conjugate
Unconjugated
CloneNo.
3N6
Cat no : 80340-1-RR
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | NIH/3T3 cells, HeLa cells, Calyculin A treated HeLa cells, Calyculin A treated HEK-293 cells, Calyculin A treated NIH/3T3 cells |
| Positive FC detected in | Calyculin A treated Hela cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Flow Cytometry (FC) | FC : 0.50 ug per 10^6 cells in a 100 µl suspension |
| Sample-dependent, check data in validation data gallery | |
Product Information
80340-1-RR targets Phospho-Hamartin/TSC1 (Ser511) in WB, FC, ELISA applications and shows reactivity with Human, mouse samples.
| Tested Reactivity | Human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | tuberous sclerosis 1 |
| Calculated molecular weight | 130 kDa |
| Observed molecular weight | 150 kDa |
| GenBank accession number | NM_000368 |
| Gene symbol | TSC1 |
| Gene ID (NCBI) | 7248 |
| RRID | AB_2918889 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TSC1, also named as Hamartin, is a tumor suppressor gene syndrome whose manifestations can include seizures, mental retardation, autism, and tumors in the brain, retina, kidney, heart, and skin. The tuberous sclerosis 1 (TSC1)/TSC2 tumor suppressor complex serves as a repressor of the mTOR pathway, and disruption of TSC1/TSC2 complex function may contribute to tumorigenesis. TSC1 is phosphorylated by IKKb at Ser487 and Ser511 in vitro and in vivo, and phosphorylation at these sites enhances dissociation of the TSC1/TSC2 complex, which in turn induces mTOR activation. (PMID: 17693255). TSC1 is also threonine-phosphorylated at three sites (Thr417, Ser584, and Thr1047) in a reaction catalyzed by cyclin-dependent kinase 1 (CDK1) (PMID: 14551205).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-Hamartin/TSC1 (Ser511) antibody 80340-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
