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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-GYS1 (Ser641) Polyclonal antibody

Phospho-GYS1 (Ser641) Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 28855-1-AP

Synonyms

glycogen synthase 1 (muscle), GSY, GYS, GYS1, Phospho-GYS1 (Ser641)

Validation Data Gallery

Applications:
  • WB analysis using 28855-1-AP

    WB analysis using 28855-1-AP

    Non-treated PC-3 cells, phosphatase inhibitor treated and λ phosphatase PC-3 cells were subjected to SDS PAGE followed by western blot with 28855-1-AP (Phospho-GYS1 (Ser641) antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

  • IF Staining of PC-3 using 28855-1-AP

    IF Staining of PC-3 using 28855-1-AP

    Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated PC-3 cells using Phospho-GYS1 (Ser641) antibody (28855-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inλ phosphatase treated PC-3 cells
Positive IF detected inλ phosphatase treated PC-3 cells
Planning an IHC experiment? We recommend our IHCeasy GYS1 Ready-To-Use IHC Kit. GYS1 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

28855-1-AP targets Phospho-GYS1 (Ser641) in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name glycogen synthase 1 (muscle)
Calculated molecular weight 84 kDa
Observed molecular weight84 kDa
GenBank accession numberBC007688
Gene symbol GYS1
Gene ID (NCBI) 2997
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glycogen synthase 1 (GYS1, GS) catalyzes the key step of glycogen synthesis and plays an important role in glycogen metabolism in liver and muscle. In kidney tissues, glycogen synthase 1 (GYS1) is the most important rate-limiting enzyme functioning in the last step of glycogen synthesis. Pathologically, its deficiency has been shown to cause muscle glycogen storage disease type 0 and death. Studies of tumors showed that GYS1 was rapidly induced under hypoxic conditions and positively correlated with glycogen accumulation in glioblastoma, breast, and colon cancer cell lines. GYS1 is phosphorylated at nine sites and insulin stimulates dephosphorylation of glycogen synthase. Insulin stimulates dephosphorylation of glycogen synthase via PKB-mediated phosphorylation of GSK3. Phosphorylation of GSK3 decreases kinase activity which will decrease phosphorylation of GS and increase glycogens synthase fractional activity. (PMID: 32802186, PMID: 30443599, PMID: 22232606)

Protocols

Product Specific Protocols
WB protocol for Phospho-GYS1 (Ser641) antibody 28855-1-APDownload protocol
IF protocol for Phospho-GYS1 (Ser641) antibody 28855-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols