Validation Data Gallery
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Tested Applications
| Positive WB detected in | RAW 264.7 cells |
| Positive IHC detected in | human breast cancer tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MDCK cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 6 publications below |
| IHC | See 1 publications below |
Product Information
22172-1-AP targets Paxillin in WB, IF, IHC, ELISA applications and shows reactivity with human, Canine, mouse samples.
| Tested Reactivity | human, Canine, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17702 Product name: Recombinant human PXN protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-267 aa of BC136787 Sequence: MDDLDALLADLESTTSHISKRPVFLSEETPYSYPTGNHTYQEIAVPPPVPPPPSSEALNGTILDPLDQWQPSGSRFIHQQPQSSSPVYGSSAKTSSVSNPQDSVGSPCSRVGEEEHVYSFPNKQKSAEPSPTVMSTSLGSNLSELDRLLLELNAVQHNPPGFPADEANSSPPLPGALSPLYGVPETNSPLGGKAGPLTKEKPKRNGGRGLEDVRPSVESLLDELESSVPSPVPAITVNQGEMSSPQRVTSTQQQTRISASSATRELD 相同性解析による交差性が予測される生物種 |
| Full Name | paxillin |
| Calculated molecular weight | 591 aa, 65 kDa |
| GenBank accession number | BC136787 |
| Gene Symbol | Paxillin |
| Gene ID (NCBI) | 5829 |
| RRID | AB_2879016 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P49023 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Paxillin antibody 22172-1-AP | Download protocol |
| IHC protocol for Paxillin antibody 22172-1-AP | Download protocol |
| WB protocol for Paxillin antibody 22172-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oncogene KIF26B, a novel oncogene, promotes proliferation and metastasis by activating the VEGF pathway in gastric cancer. | ||
Sci Rep A novel cystathionine γ-lyase inhibitor, I194496, inhibits the growth and metastasis of human TNBC via downregulating multiple signaling pathways. | ||
Sci Rep Interferon-inducible protein, IFIX, has tumor-suppressive effects in oral squamous cell carcinoma | ||
Int J Oncol Cystathionine‑γ‑lyase promotes the metastasis of breast cancer via the VEGF signaling pathway. | ||
Cell Death Discov A novel chemical inhibitor suppresses breast cancer cell growth and metastasis through inhibiting HPIP oncoprotein. | ||
Cell Signal Activated CRH receptors inhibit autophagy by repressing conversion of LC3BI to LC3BII. |
