Parvalbumin Polyclonal antibody
Parvalbumin Polyclonal Antibody for IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse, rat
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 29312-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive IHC detected in | mouse cerebellum tissue, mouse brain tissue, rat cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | mouse cerebellum tissue, mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 1 publications below |
| IF | See 2 publications below |
Product Information
29312-1-AP targets Parvalbumin in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Parvalbumin fusion protein Ag30189 相同性解析による交差性が予測される生物種 |
| Full Name | parvalbumin |
| Calculated molecular weight | 12 kDa |
| GenBank accession number | NM_002854 |
| Gene symbol | PVALB |
| Gene ID (NCBI) | 5816 |
| RRID | AB_2918280 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PVALB is a high affinity calcium ion-binding protein that is structurally and functionally similar to calmodulin and troponin C. PVALB is expressed in high levels only in fast-contracting muscles and at lower levels in brain and several endocrine tissues. It is thought to be involved in muscle relaxation.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Parvalbumin antibody 29312-1-AP | Download protocol |
| IF protocol for Parvalbumin antibody 29312-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Rep Methods A versatile vessel casting method for fine mapping of vascular networks using a hydrogel-based lipophilic dye solution | ||
STAR Protoc Protocol for fine casting, imaging, and analysis of murine vascular networks with VALID | ||
Sci Rep Identification of mouse soleus muscle proteins altered in response to changes in gravity loading |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Reyes (Verified Customer) (03-01-2024) | Parvalbumin (red) antibody worked in my human brain cortex sections, although it marks mostly the synaptic terminals more than the soma of the parvalbumin cells
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