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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PYCARD Polyclonal antibody

PYCARD Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30641-1-AP

Synonyms

9130417A21Rik, Asc, CARD5, masc, Pycard, PYD and CARD domain containing, TMS 1, TNS1

Validation Data Gallery

Applications:
  • WB analysis using 30641-1-AP

    WB analysis using 30641-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30641-1-AP (PYCARD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse spleen tissue, mouse thymus tissue, rat spleen tissue, rat thymus tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

Product Information

30641-1-AP targets PYCARD in WB, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PYCARD fusion protein Ag32519 相同性解析による交差性が予測される生物種
Full Name PYD and CARD domain containing
Calculated molecular weight21KD
Observed molecular weight21-25 kDa
GenBank accession numberNM_023258
Gene symbol Pycard
Gene ID (NCBI) 66824
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PYCARD (PYD and CARD domain containing), also known as ASC and CARD5. The calculated molecular weight of PYCARD is 21 kDa. It is located in cytosol, extracellular region and nucleus. PYCARD is also expressed in several structures, including alimentary system; brain; heart; hemolymphoid system gland; and urinary system. The gene enables identical protein binding activity and protein dimerization activity. Involved in several processes, including activation of cysteine-type endopeptidase activity; positive regulation of cytokine production; and regulation of defense response. It acts upstream of or within several processes, including defense response to Gram-positive bacterium; positive regulation of macromolecule metabolic process; and regulation of autophagy. It is also a part of inflammasome complex. Finally, it orthologous to human PYCARD (PYD and CARD domain containing).

Protocols

Product Specific Protocols
WB protocol for PYCARD antibody 30641-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols