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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PTPRZ1 Polyclonal antibody

PTPRZ1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55125-1-AP

Synonyms

HPTPZ, HPTPzeta, HTPZP2, phosphacan, PTP ZETA, PTP18, PTPRZ, PTPRZ1, PTPRZ2, PTPZ, R PTP zeta, R PTP zeta 2, RPTPB, RPTPbeta

Validation Data Gallery

Applications:
  • WB analysis of HepG2 using 55125-1-AP

    WB analysis of HepG2 using 55125-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 55125-1-AP (PTPRZ1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 55125-1-AP

    WB analysis of HeLa using 55125-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 55125-1-AP (PTPRZ1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of rat cerebellum using 55125-1-AP

    IHC staining of rat cerebellum using 55125-1-AP

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 55125-1-AP (PTPRZ1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat cerebellum using 55125-1-AP

    IHC staining of rat cerebellum using 55125-1-AP

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 55125-1-AP (PTPRZ1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHepG2 cells, HeLa cells
Positive IHC detected inrat cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

55125-1-AP targets PTPRZ1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor-type, Z polypeptide 1
Calculated molecular weight 255 kDa
Observed molecular weight 163-175 kDa
GenBank accession numberNM_002851
Gene symbol PTPRZ1
Gene ID (NCBI) 5803
RRIDAB_10858795
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PTPRZ1, also named as HTPZP2, PTPRZ, PTPRZ2 and PTPZ, belongs to the protein-tyrosine phosphatase family and Receptor class 5 subfamily. It may be involved in the regulation of specific developmental processes in the CNS. PTPRZ1 has some isoforms with MW 254 kDa, 163 kDa and 90 kDa.

Protocols

Product Specific Protocols
WB protocol for PTPRZ1 antibody 55125-1-APDownload protocol
IHC protocol for PTPRZ1 antibody 55125-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Clin Invest

Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling.

Authors - Zhixiong Liu
View Article
humanWB,IHC

J Oncol

Overexpression of PTPRZ1 Regulates p120/β-Catenin Phosphorylation to Promote Carcinogenesis of Oral Submucous Fibrosis.

Authors - Liwei Ma
View Article