IA-2/PTPRN Monoclonal antibody

IA-2/PTPRN Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG3

Reactivity

human

Applications

WB, IHC, IF

Conjugate

Unconjugated

CloneNo.

1D3F5

Cat no : 66045-1-Ig

Synonyms

IA 2/PTP, IA2, IA-2, ICA 512, ICA3, ICA512, Islet cell antigen 512, Islet cell autoantigen 3, PTP IA 2, PTPRN, R PTP N



Tested Applications

Positive WB detected inHuman brain, tissue
Positive IHC detected inhuman pancreas cancer tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66045-1-Ig is IA-2/PTPRN Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen IA-2/PTPRN fusion protein Ag17909
Full Name protein tyrosine phosphatase, receptor type, N
Calculated molecular weight 105 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC007713
Gene symbol PTPRN
Gene ID (NCBI) 5798
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IA-2/PTPRN is a member of the protein tyrosine phosphatase (PTP) family, which contains a transmembrane region, an intracellular PTP-like domain and an extracellular N-terminus. Experiments found that IA-2/PTPRN localizes to secretory granules and is exclusively expressed in neuroendocrine cells (including pancreatic islets cell). IA-2/PTPRNwas found to be a major autoantigen in insulin-dependent diabetes mellitus and the detection of autoantibodies against IA-2/PTPRN is commonly used as a diabetes diagnosis marker.

Protocols

Product Specific Protocols
WB protocol for IA-2/PTPRN antibody 66045-1-IgDownload protocol
IHC protocol for IA-2/PTPRN antibody 66045-1-IgDownload protocol
IF protocol for IA-2/PTPRN antibody 66045-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols