CoraLite® Plus 488-conjugated PTPN6 Polyclonal antibody
PTPN6 Polyclonal Antibody for IF, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IF, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
Cat no : CL488-24546
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive IF detected in | MCF-7 cells |
| Positive FC detected in | MCF-7 cells |
For other applications, we recommend the unconjugated version of this antibody, 24546-1-AP
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Sample-dependent, check data in validation data gallery | |
Product Information
CL488-24546 targets PTPN6 in IF, FC (Intra) applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PTPN6 fusion protein Ag21415 相同性解析による交差性が予測される生物種 |
| Full Name | protein tyrosine phosphatase, non-receptor type 6 |
| Calculated molecular weight | 597 aa, 68 kDa |
| Observed molecular weight | 63 kDa |
| GenBank accession number | BC002523 |
| Gene symbol | PTPN6 |
| Gene ID (NCBI) | 5777 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PTPN6(Tyrosine-protein phosphatase non-receptor type 6) is also named as HCP, PTP1C and belongs to the protein-tyrosine phosphatase family. It regulates muscle INS action in a cell-autonomous manner, further suggesting that the PTPase negatively modulates INS action through down-regulation of both INS signaling to AKT1 and SLC2A4 translocation, as well as SLC2A4 expression(PMID:21952243). It has 4 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 PTPN6 antibody CL488-24546 | Download protocol |
| FC protocol for CL Plus 488 PTPN6 antibody CL488-24546 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
