Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 24546-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 24546-1-AP (PTPN6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 24546-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 24546-1-AP (SHP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HuH-7 using 24546-1-AP
HuH-7 cells were subjected to SDS PAGE followed by western blot with 24546-1-AP (SHP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IP experiment of Jurkat using 24546-1-AP
IP result of anti-PTPN6 (IP:24546-1-AP, 4ug; Detection:24546-1-AP 1:600) with Jurkat cells lysate 3600ug.
IHC staining of human colon cancer using 24546-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24546-1-AP (PTPN6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of MCF-7 using 24546-1-AP
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PTPN6 antibody (24546-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of MCF-7 using 24546-1-AP
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PTPN6 antibody (24546-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of MCF-7 using 24546-1-AP
1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human PTPN6 (24546-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Featured Product
KD/KO Validated

PTPN6/SHP1 Polyclonal antibody

PTPN6/SHP1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 24546-1-AP

Publications(19)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

PTPN6, SHP1, EC:3.1.3.48, HCP, HCPH

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (19)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Raji cells, Jurkat cells, HuH-7 cells
Positive IP detected in	Jurkat cells
Positive IHC detected in	human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells
Positive FC (Intra) detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 17 publications below
IHC	See 1 publications below
IF	See 2 publications below
IP	See 2 publications below
CoIP	See 2 publications below

Product Information

24546-1-AP targets PTPN6/SHP1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag21415 Product name: Recombinant human PTPN6 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 506-598 aa of BC002523 Sequence: QYKFIYVAIAQFIETTKKKLEVLQSQKGQESEYGNITYPPAMKNAHAKASRTSSKHKEDVYENLHTKNKREEKVKKQRSADKEKSKGSLKRK 相同性解析による交差性が予測される生物種
Full Name	protein tyrosine phosphatase, non-receptor type 6
Calculated molecular weight	597 aa, 68 kDa
Observed molecular weight	63 kDa
GenBank accession number	BC002523
Gene Symbol	PTPN6/SHP1
Gene ID (NCBI)	5777
RRID	AB_2879600
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P29350
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PTPN6(Tyrosine-protein phosphatase non-receptor type 6) is also named as HCP, PTP1C and belongs to the protein-tyrosine phosphatase family. It regulates muscle INS action in a cell-autonomous manner, further suggesting that the PTPase negatively modulates INS action through down-regulation of both INS signaling to AKT1 and SLC2A4 translocation, as well as SLC2A4 expression(PMID:21952243). It has 4 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
IF protocol for PTPN6/SHP1 antibody 24546-1-AP	Download protocol
IHC protocol for PTPN6/SHP1 antibody 24546-1-AP	Download protocol
IP protocol for PTPN6/SHP1 antibody 24546-1-AP	Download protocol
WB protocol for PTPN6/SHP1 antibody 24546-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IF	Elife Molecular features underlying differential SHP1/SHP2 binding of immune checkpoint receptors. Authors - Xiaozheng Xu View Article
human	WB	Oncotarget Dietary quercetin potentiates the antiproliferative effect of interferon-α in hepatocellular carcinoma cells through activation of JAK/STAT pathway signaling by inhibition of SHP2 phosphatase. Authors - Ighodaro Igbe View Article
mouse,human	WB	Front Immunol Host MKRN1-Mediated Mycobacterial PPE Protein Ubiquitination Suppresses Innate Immune Response. Authors - Yafeng Dou KD Validated View Article
human	WB	Int J Biol Sci PMID: 29805309 Authors - Linna Xie View Article
human	WB	Oxid Med Cell Longev Proscillaridin A Promotes Oxidative Stress and ER Stress, Inhibits STAT3 Activation, and Induces Apoptosis in A549 Lung Adenocarcinoma Cells. Authors - Amara Maryam View Article
human	WB	J Biol Chem A thiazole-derived oridonin analog exhibits antitumor activity by directly and allosterically inhibiting STAT3. Authors - Xiaofei Shen View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 24546-1-AP

WB analysis using 24546-1-AP

WB analysis of HuH-7 using 24546-1-AP

IP experiment of Jurkat using 24546-1-AP

IHC staining of human colon cancer using 24546-1-AP

IF Staining of MCF-7 using 24546-1-AP

IF Staining of MCF-7 using 24546-1-AP

FC experiment of MCF-7 using 24546-1-AP

PTPN6/SHP1 Polyclonal antibody

PTPN6/SHP1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Molecular features underlying differential SHP1/SHP2 binding of immune checkpoint receptors.

Dietary quercetin potentiates the antiproliferative effect of interferon-α in hepatocellular carcinoma cells through activation of JAK/STAT pathway signaling by inhibition of SHP2 phosphatase.

Host MKRN1-Mediated Mycobacterial PPE Protein Ubiquitination Suppresses Innate Immune Response.

PMID: 29805309

Proscillaridin A Promotes Oxidative Stress and ER Stress, Inhibits STAT3 Activation, and Induces Apoptosis in A549 Lung Adenocarcinoma Cells.

A thiazole-derived oridonin analog exhibits antitumor activity by directly and allosterically inhibiting STAT3.