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Validation Data Gallery

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  • Western Blot (WB) analysis of L02 cells using PTH2R Polyclonal antibody (14166-1-AP)

    WB analysis of L02 using 14166-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 14166-1-AP (PTH2R antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using PTH2R Polyclonal antibody (14166-1-AP)

    WB analysis of mouse testis using 14166-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 14166-1-AP (PTH2R antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using PTH2R Polyclonal antibody (14166-1-AP)

    WB analysis of human testis using 14166-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 14166-1-AP (PTH2R antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using PTH2R Polyclonal antibody (14166-1-AP)

    WB analysis of mouse liver using 14166-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 14166-1-AP (PTH2R antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of BxPC-3 cells using PTH2R Polyclonal antibody (14166-1-AP)

    WB analysis of BxPC-3 using 14166-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14166-1-AP (PTH2R antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse pancreas tissue using PTH2R Polyclonal antibody (14166-1-AP)

    WB analysis of mouse pancreas using 14166-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14166-1-AP (PTH2R antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using PTH2R Polyclonal antibody (14166-1-AP)

    IHC staining of human pancreas cancer using 14166-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14166-1-AP (PTH2R antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using PTH2R Polyclonal antibody (14166-1-AP)

    IHC staining of human pancreas cancer using 14166-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14166-1-AP (PTH2R antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using PTH2R Polyclonal antibody (14166-1-AP)

    IHC staining of human renal cell carcinoma using 14166-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 14166-1-AP (PTH2R antibody) at dilution of 1:25 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using PTH2R Polyclonal antibody (14166-1-AP)

    IHC staining of human renal cell carcinoma using 14166-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 14166-1-AP (PTH2R antibody) at dilution of 1:25 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using PTH2R Polyclonal antibody (14166-1-AP)

    IHC staining of human renal cell carcinoma using 14166-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 14166-1-AP (PTH2R antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using PTH2R Polyclonal antibody (14166-1-AP)

    IHC staining of human renal cell carcinoma using 14166-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 14166-1-AP (PTH2R antibody) at dilution of 1:50 (under 40x lens).

  • Flow cytometry (FC) experiment of BxPC-3 cells using PTH2R Polyclonal antibody (14166-1-AP)

    FC experiment of BxPC-3 using 14166-1-AP

    1x10^6 BxPC-3 cells were intracellularly stained with 0.4 ug PTH2R Polyclonal antibody (14166-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

PTH2R Polyclonal antibody

PTH2R Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14166-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

parathyroid hormone 2 receptor, PTH2 receptor, PTHR2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inL02 cells, BxPC-3 cells, human testis tissue, mouse liver tissue, mouse pancreas tissue, mouse testis tissue
Positive IHC detected inhuman pancreas cancer tissue, human renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inBxPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

14166-1-AP targets PTH2R in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5364

Product name: Recombinant human PTH2R protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 419-550 aa of BC036811

Sequence: EVQAEVKKMWSRWNLSVDWKRTPPCGSRRCGSVLTTVTHSTSSQSQVAASTRMVLISGKAAKIASRQPDSHITLPGYVWSNSEQDCLPHSFHEETKEDSGRQGDDILMEKPSRPMESNPDTEGCQGETEDVL

相同性解析による交差性が予測される生物種
Full Name parathyroid hormone 2 receptor
Calculated molecular weight 550 aa, 62 kDa
Observed molecular weight 62-66 kDa
GenBank accession numberBC036811
Gene Symbol PTH2R
Gene ID (NCBI) 5746
RRIDAB_2253212
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP49190
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Parathyroid hormone 2 receptor (PTH2R, also known as PTHR2) is a member of the secretin receptor family of G protein-coupled receptors. PTH2R selectively recognizes parathyroid hormone and is particularly abundant in the brain and pancreas (PMID: 7797535). Tuberoinfundibular peptide of 39 residues (TIP39) is the endogenous ligand of the PTH2R in the brain, and they form a neuromodulator system (PMID: 19857544). PTH2R is involved in the regulation of calcium transport, nociception mediation, and wound healing (PMID: 34353904).

Protocols

Product Specific Protocols
FC protocol for PTH2R antibody 14166-1-APDownload protocol
IHC protocol for PTH2R antibody 14166-1-APDownload protocol
WB protocol for PTH2R antibody 14166-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Hematol

Neuropeptide TIP39 induces autophagy in PTH2 receptor-positive myeloid neoplasms

Authors - Kento Ono
View Article
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