Validation Data Gallery
Tested Applications
| Positive WB detected in | rat brain tissue, HL-60 cells, mouse brain tissue |
| Positive IHC detected in | human prostate hyperplasia tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 16 publications below |
| IHC | See 2 publications below |
| IF | See 7 publications below |
Product Information
24895-1-AP targets PTGER4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19217 Product name: Recombinant human PTGER4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 333-488 aa of BC101534 Sequence: RKTVLSKAIEKIKCLFCRIGGSRRERSGQHCSDSQRTSSAMSGHSRSFISRELKEISSTSQTLLPDLSLPDLSENGLGGRNLLPGVPGMGLAQEDTTSLRTLRISETSDSSQGQDSESVLLVDEAGGSGRAGPAPKGSSLQVTFPSETLNLSEKCI 相同性解析による交差性が予測される生物種 |
| Full Name | prostaglandin E receptor 4 (subtype EP4) |
| Calculated molecular weight | 488 aa, 53 kDa |
| Observed molecular weight | 53 kDa |
| GenBank accession number | BC101534 |
| Gene Symbol | PTGER4 |
| Gene ID (NCBI) | 5734 |
| RRID | AB_2879782 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P35408 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PTGER4 (Prostaglandin E2 receptor EP4 subtype, also known as EP4R) is a member of the G-protein coupled receptor family. This protein is one of four receptors identified for prostaglandin E2 (PGE2). May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function.
Publications
| Species | Application | Title |
|---|---|---|
Theranostics Abnormal mitochondrial iron metabolism damages alveolar type II epithelial cells involved in bleomycin-induced pulmonary fibrosis | ||
J Clin Invest Antibiotic pretreatment alleviates liver transplant damage in mice and humans. | ||
Adv Sci (Weinh) Endogenous Ceramide 24:1 Constrains Th17-Driven Neutrophilic Inflammation by Antagonizing EP2 Signaling. | ||
Cell Commun Signal PTGER4 signaling regulates class IIa HDAC function and SPINK4 mRNA levels in rectal epithelial cells | ||
J Orthop Translat Sensory nerve EP4 facilitates heterotopic ossification by regulating angiogenesis-coupled bone formation | ||
Mol Med Hyperoxia-activated Nrf2 regulates ferroptosis in intestinal epithelial cells and intervenes in inflammatory reaction through COX-2/PGE2/EP2 pathway |