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PTBP1 Monoclonal antibody
PTBP1 Monoclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
3D6D8
Cat no : 67462-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, LNCaP cells, HEK-293 cells. K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells |
| Positive IHC detected in | human colon cancer tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | A375 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
Product Information
67462-1-Ig targets PTBP1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | PTBP1 fusion protein Ag28404 相同性解析による交差性が予測される生物種 |
| Full Name | polypyrimidine tract binding protein 1 |
| Calculated molecular weight | 60 kDa |
| Observed molecular weight | 60 kDa |
| GenBank accession number | BC013694 |
| Gene symbol | PTBP1 |
| Gene ID (NCBI) | 5725 |
| RRID | AB_2882692 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Polypyrimidine tract-binding protein (PTBP1), also known as heterogeneous nuclear ribonucleoprotein type I (hnRNP I), is a nuclear protein that binds pre-mRNAs in specific regions of the hnRNA-protein complexes sensitive to micrococcal nuclease. It involves in pre-mRNA splicing and the regulation of alternative splicing events. PTBP1 promotes RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA, and promotes the binding of U2 snRNP to pre-mRNA. Besides, it can cooperate with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA, and represses the splicing of MAPT/Tau exon 10.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PTBP1 antibody 67462-1-Ig | Download protocol |
| IHC protocol for PTBP1 antibody 67462-1-Ig | Download protocol |
| IF protocol for PTBP1 antibody 67462-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Toxicol Sci Propofol improves ischemia reperfusion-induced liver fibrosis by regulating lncRNA HOXA11-AS
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