Validation Data Gallery

Filter: AllWBIHCIF-P

WB analysis of HeLa using 12582-1-AP
WB result of PTBP1 antibody (12582-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTBP1 transfected HeLa cells.
WB analysis using 12582-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12582-1-AP (PTBP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of rat heart using 12582-1-AP
rat heart tissue were subjected to SDS PAGE followed by western blot with 12582-1-AP (PTBP1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 12582-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 12582-1-AP (PTBP1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC staining of mouse brain using 12582-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12582-1-AP (PTBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 12582-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12582-1-AP (PTBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 12582-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12582-1-AP (PTBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 12582-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12582-1-AP (PTBP1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human pancreas using 12582-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas slide using 12582-1-AP (PTBP1 Antibody) at dilution of 1:50.
IHC staining of human pancreas using 12582-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas slide using 12582-1-AP (PTBP1 Antibody) at dilution of 1:50.
IHC staining of mouse brain using 12582-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12582-1-AP (PTBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse brain using 12582-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 12582-1-AP (PTBP1 antibody), at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse brain using 12582-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 12582-1-AP (PTBP1 antibody), at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

PTBP1 Polyclonal antibody

PTBP1 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 12582-1-AP

Publications(53)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, CoIP, RIP, ELISA

Synonyms

PTB 1, PTB, pPTB, HNRPI, HNRNPI

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (53)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A431 cells, HeLa cells, rat heart tissue, mouse lung tissue, BxPC-3 cells, Jurkat cells, K-562 cells
Positive IHC detected in	human prostate cancer tissue, human pancreas tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse brain tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 11 publications below
WB	See 41 publications below
IHC	See 4 publications below
IF	See 11 publications below
IP	See 4 publications below
CoIP	See 2 publications below
RIP	See 5 publications below

Product Information

12582-1-AP targets PTBP1 in WB, IHC, IF-P, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3277 Product name: Recombinant human PTBP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-370 aa of BC013694 Sequence: MDGIVPDIAVGTKRGSDELFSTCVTNGPFIMSSNSASAANGNDSKKFKGDSRSAGVPSRVIHIRKLPIDVTEGEVISLGLPFGKVTNLLMLKGKNQAFIEMNTEEAANTMVNYYTSVTPVLRGQPIYIQFSNHKELKTDSSPNQARAQAALQAVNSVQSGNLALAASAAAVDAGMAMAGQSPVLRIIVENLFYPVTLDVLHQIFSKFGTVLKIITFTKNNQFQALLQYADPVSAQHAKLSLDGQNIYNACCTLRIDFSKLTSLNVKYNNDKSRDYTRPDLPSGDSQPSLDQTMAAAFGAPGIISASPYAGAGFPPTFAIPQAAGLSVPNVHGALAPLAIPSAAAAAAAAGRIAIPGLAGAGNSVLLVSNL 相同性解析による交差性が予測される生物種
Full Name	polypyrimidine tract binding protein 1
Calculated molecular weight	60 kDa
Observed molecular weight	60 kDa
GenBank accession number	BC013694
Gene Symbol	PTBP1
Gene ID (NCBI)	5725
RRID	AB_2176402
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P26599
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Polypyrimidine tract-binding protein (PTBP1), also known as heterogeneous nuclear ribonucleoprotein type I (hnRNP I), is a nuclear protein that binds pre-mRNAs in specific regions of the hnRNA-protein complexes sensitive to micrococcal nuclease. It involves in pre-mRNA splicing and the regulation of alternative splicing events. PTBP1 promotes RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA, and promotes the binding of U2 snRNP to pre-mRNA. Besides, it can cooperate with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA, and represses the splicing of MAPT/Tau exon 10

Protocols

Product Specific Protocols
IF protocol for PTBP1 antibody 12582-1-AP	Download protocol
IHC protocol for PTBP1 antibody 12582-1-AP	Download protocol
WB protocol for PTBP1 antibody 12582-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Cancer SULT1A1-dependent sulfonation of alkylators is a lineage-dependent vulnerability of liver cancers Authors - Lei Shi View Article
human	WB	Nat Cell Biol Arg-tRNA synthetase links inflammatory metabolism to RNA splicing and nuclear trafficking via SRRM2 Authors - Haissi Cui View Article
human	RIP	Blood circMYBL2, a circRNA from MYBL2, regulates FLT3 translation by recruiting PTBP1 to promote FLT3-ITD AML progression. Authors - Yu-Meng Sun View Article
human	WB	Nat Commun Systematic characterization of cancer transcriptome at transcript resolution Authors - Wei Hu View Article
rat	WB	Nucleic Acids Res PMID: 30295850 Authors - Zonghong Li View Article
human	WB	Dev Cell Genome-wide analysis of pseudogenes reveals HBBP1's human-specific essentiality in erythropoiesis and implication in β-thalassemia. Authors - Yanni Ma View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 12582-1-AP

WB analysis using 12582-1-AP

WB analysis of rat heart using 12582-1-AP

WB analysis of mouse lung using 12582-1-AP

IHC staining of mouse brain using 12582-1-AP

IHC staining of human prostate cancer using 12582-1-AP

IHC staining of human prostate cancer using 12582-1-AP

IHC staining of human prostate cancer using 12582-1-AP

IHC staining of human pancreas using 12582-1-AP

IHC staining of human pancreas using 12582-1-AP

IHC staining of mouse brain using 12582-1-AP

IF Staining of mouse brain using 12582-1-AP

IF Staining of mouse brain using 12582-1-AP

PTBP1 Polyclonal antibody

PTBP1 Polyclonal Antibody for WB, IHC, IF-P, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

SULT1A1-dependent sulfonation of alkylators is a lineage-dependent vulnerability of liver cancers

Arg-tRNA synthetase links inflammatory metabolism to RNA splicing and nuclear trafficking via SRRM2

circMYBL2, a circRNA from MYBL2, regulates FLT3 translation by recruiting PTBP1 to promote FLT3-ITD AML progression.

Systematic characterization of cancer transcriptome at transcript resolution

PMID: 30295850

Genome-wide analysis of pseudogenes reveals HBBP1's human-specific essentiality in erythropoiesis and implication in β-thalassemia.