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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PSMB3 Polyclonal antibody

PSMB3 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15983-1-AP

Synonyms

20S proteasome, HC10 II, Proteasome chain 13, Proteasome component C10 II, Proteasome subunit beta type 3, Proteasome theta chain, PSMB3

Validation Data Gallery

Applications:
  • WB analysis using 15983-1-AP

    WB analysis using 15983-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15983-1-AP (PSMB3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 15983-1-AP

    WB analysis using 15983-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15983-1-AP (PSMB3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 15983-1-AP

    WB analysis using 15983-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 15983-1-AP (PSMB3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 15983-1-AP

    IHC staining of human liver using 15983-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15983-1-AP (PSMB3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMB3 antibody included
  • IHC staining of human liver using 15983-1-AP

    IHC staining of human liver using 15983-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15983-1-AP (PSMB3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMB3 antibody included
  • IF Staining of Jurkat using 15983-1-AP

    IF Staining of Jurkat using 15983-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using PSMB3 antibody (15983-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inJurkat cells, HEK-293T cells, mouse testis tissue, rat testis tissue, HeLa cells, MCF-7 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy PSMB3 Ready-To-Use IHC Kit. PSMB3 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15983-1-AP targets PSMB3 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSMB3 fusion protein Ag8784 相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) subunit, beta type, 3
Calculated molecular weight 205 aa, 23 kDa
Observed molecular weight 23-27 kDa
GenBank accession numberBC013008
Gene symbol PSMB3
Gene ID (NCBI) 5691
RRIDAB_2172027
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PSMB3 antibody 15983-1-APDownload protocol
IHC protocol for PSMB3 antibody 15983-1-APDownload protocol
IF protocol for PSMB3 antibody 15983-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols