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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated PSMB1 Monoclonal antibody

PSMB1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1C7C5

Cat no : CL594-67847

Synonyms

FLJ25321, HC5, KIAA1838, Macropain subunit C5, PMSB1, Proteasome component C5, Proteasome gamma chain, Proteasome subunit beta type 1, PSC5, PSMB1

Validation Data Gallery

Applications:
  • FC experiment of HeLa using CL594-67847

    FC experiment of HeLa using CL594-67847

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human PSMB1 (CL594-67847, Clone:1C7C5) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PSMB1 Ready-To-Use IHC Kit. PSMB1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67847-1-Ig

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL594-67847 targets PSMB1 in FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PSMB1 fusion protein Ag30791 相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) subunit, beta type, 1
Calculated molecular weight 241 aa, 26 kDa
Observed molecular weight25 kDa
GenBank accession numberBC020807
Gene symbol PSMB1
Gene ID (NCBI) 5689
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PSMB1(Proteasome subunit beta type-1) is also named as PSC5 and belongs to the peptidase T1B family. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The gene encodes a 241 amino acid protein with a 28 amino acid propeptide and two glycosylation sites.

Protocols

Product Specific Protocols
FC protocol for CL594 PSMB1 antibody CL594-67847Download protocol
Standard Protocols
Click here to view our Standard Protocols