Validation Data Gallery
Tested Applications
| Positive WB detected in | BxPC-3 cells, mouse kidney tissue, mouse spleen tissue, HeLa cells, K-562 cells, U-937 cells |
| Positive IP detected in | BxPC-3 cells |
| Positive IHC detected in | human pancreas cancer tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IHC | See 3 publications below |
Product Information
11943-2-AP targets PSMA4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2542 Product name: Recombinant human PSMA4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-237 aa of BC056249 Sequence: MEAIGHAGTCLGILANDGVLLAAERRNIHKLLDEVFFSEKIYKLNEDMACSVAGITSDANVLTNELRLIAQRYLLQYQEPIPCEQLVTALCDIKQAYTQFGGKRPFGVSLLYIGWDKHYGFQLYQSDPSGNYGGWKATCIGNNSAAAVSMLKQDYKEGEMTLKSALALAIKVLNKTMDVSKLSAEKVEIATLTRENGKTVIRVLKQKEVEQLIKKHEEEEAKAEREKKEKEQKEKDK 相同性解析による交差性が予測される生物種 |
| Full Name | proteasome (prosome, macropain) subunit, alpha type, 4 |
| Calculated molecular weight | 237 aa, 27 kDa |
| Observed molecular weight | 29 kDa |
| GenBank accession number | BC056249 |
| Gene Symbol | PSMA4 |
| Gene ID (NCBI) | 5685 |
| RRID | AB_874213 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P25789 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PSMA4 (Proteasome Subunit Alpha 4) is a gene that encodes the alpha-4 subunit of the 20S proteasome core particle, which forms part of the outer alpha-ring structure of the barrel-shaped 20S complex. As an integral component of the 26S proteasome, PSMA4 participates in ATP- and ubiquitin-dependent protein degradation, a process essential for the regulation of cell cycle progression, signal transduction, and protein quality control. In addition, the 20S core particle can associate with regulatory complexes such as PA28 or PA200 to mediate ubiquitin-independent protein degradation, which plays important roles in pathways including antigen peptide generation and spermatogenesis. Dysregulation of PSMA4 expression or function has been implicated in various pathological conditions, including cancer and neurodegenerative diseases, highlighting its critical role in maintaining cellular proteostasis.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PSMA4 antibody 11943-2-AP | Download protocol |
| IHC protocol for PSMA4 antibody 11943-2-AP | Download protocol |
| IP protocol for PSMA4 antibody 11943-2-AP | Download protocol |
| WB protocol for PSMA4 antibody 11943-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Rep Proteasome inhibition by bortezomib parallels a reduction in head and neck cancer cells growth, and an increase in tumor-infiltrating immune cells. | ||
PLoS One Inactive USP14 and inactive UCHL5 cause accumulation of distinct ubiquitinated proteins in mammalian cells. | ||
Cell Death Differ Quantitative reactive cysteinome profiling reveals a functional link between ferroptosis and proteasome-mediated degradation | ||
Cell Death Discov A comparative analysis of serum and tissue proteomic profiles in non-small cell lung cancer patients with or without brain metastasis. | ||
Cell Death Dis Dihydrolipoamide dehydrogenase (DLD) is a novel molecular target of bortezomib |