Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of NIH/3T3 using 14377-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14377-1-AP (PSMA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 14377-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14377-1-AP (PSMA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 14377-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 14377-1-AP (PSMA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 14377-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 14377-1-AP (PSMA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 14377-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 14377-1-AP (PSMA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 14377-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 14377-1-AP (PSMA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 14377-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 14377-1-AP (PSMA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 14377-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 14377-1-AP (PSMA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human skeletal muscle using 14377-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 14377-1-AP (PSMA2 antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human skeletal muscle using 14377-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 14377-1-AP (PSMA2 antibody) at dilution of 1:200 (under 40x lens).
IF Staining of HepG2 using 14377-1-AP
Immunofluorescent analysis of HepG2 cells using 14377-1-AP (PSMA2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

PSMA2 Polyclonal antibody

PSMA2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 14377-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

PSC3, Proteasome subunit alpha type-2, HC3

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (4)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	NIH/3T3 cells, HEK-293 cells, HepG2 cells, mouse kidney tissue, PC-3 cells
Positive IHC detected in	human skeletal muscle tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
IHC	See 1 publications below

Product Information

14377-1-AP targets PSMA2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5753 Product name: Recombinant human PSMA2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-234 aa of BC047697 Sequence: MAERGYSFSLTTFSPSGKLVQIEYALAAVAGGAPSVGIKAANGVVLATEKKQKSILYDERSVHKVEPITKHIGLVYSGMGPDYRVLVHRARKLAQQYYLVYQEPIPTAQLVQRVASVMQEYTQSGGVRPFGVSLLICGWNEGRPYLFQSDPSGAYFAWKATAMGKNYVNGKTFLEKRYNEDLELEDAIHTAILTLKESFEGQMTEDNIEVGICNEAGFRRLTPTEVKDYLAAIA 相同性解析による交差性が予測される生物種
Full Name	proteasome (prosome, macropain) subunit, alpha type, 2
Calculated molecular weight	26 kDa
Observed molecular weight	26 kDa
GenBank accession number	BC047697
Gene Symbol	PSMA2
Gene ID (NCBI)	5683
RRID	AB_2878050
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P25787
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PSMA2 (Proteasome subunit alpha type-2) is the essential subunit that contributes to the complete assembly of 20S proteasome complex, involved in the proteolytic degradation of most intracellular proteins. It is required in spermatogenesis and generation of a subset of MHC class I-presented antigenic peptides(PMID: 34711951, PMID: 27342858). Dysregulation of PSMA2 degradation leads to tumorigenesis including cervical cancer (PMID:33684886).

Protocols

Product Specific Protocols
IF protocol for PSMA2 antibody 14377-1-AP	Download protocol
IHC protocol for PSMA2 antibody 14377-1-AP	Download protocol
WB protocol for PSMA2 antibody 14377-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
	WB	EMBO Rep MAPL loss dysregulates bile and liver metabolism in mice Authors - Vanessa Goyon View Article
human	WB	Acta Biomater Pushpin-like nanozyme for plasmon-enhanced tumor targeted therapy Authors - Baofu Ma View Article
human	IHC	J Ovarian Res Development and clinical validation of a seven-gene signature based on tumor stem cell-related genes to predict ovarian cancer prognosis Authors - Guangwei Wang View Article
human	WB	J Gastroenterol Corynoline protects chronic pancreatitis via binding to PSMA2 and alleviating pancreatic fibrosis Authors - Pengyuan Wang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of NIH/3T3 using 14377-1-AP

WB analysis of mouse kidney using 14377-1-AP

WB analysis of HEK-293 using 14377-1-AP

WB analysis of HEK-293 using 14377-1-AP

WB analysis of HepG2 using 14377-1-AP

WB analysis of HepG2 using 14377-1-AP

WB analysis of PC-3 using 14377-1-AP

WB analysis of PC-3 using 14377-1-AP

IHC staining of human skeletal muscle using 14377-1-AP

IHC staining of human skeletal muscle using 14377-1-AP

IF Staining of HepG2 using 14377-1-AP