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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated PSMA/GCPII Monoclonal antibody

PSMA/GCPII Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3G4E12

Cat no : CL594-66678

Synonyms

FGCP, Folate hydrolase 1, FOLH, FOLH1, GCP2, GCPII, Glutamate carboxypeptidase 2, Glutamate carboxypeptidase II, mGCP, NAALAD1, NAALAdase, NAALADase I, PSM, PSMA, PSMA/GCPII

Validation Data Gallery

Applications:
  • IF Staining of human prostate cancer using CL594-66678

    IF Staining of human prostate cancer using CL594-66678

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using CoraLite®594 PSMA/GCPII antibody (CL594-66678, Clone: 3G4E12 ) at dilution of 1:200.

  • IF Staining of PC-3 using CL594-66678

    IF Staining of PC-3 using CL594-66678

    Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using CoraLite®594 PSMA/GCPII antibody (CL594-66678, Clone: 3G4E12 ) at dilution of 1:200.

  • FC experiment of LNCaP using CL594-66678

    FC experiment of LNCaP using CL594-66678

    1X10^6 LNCaP cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human PSMA/GCPII (CL594-66678, Clone:3G4E12) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inPC-3 cells, human prostate cancer tissue
Positive FC detected inLNCaP cells
For other applications, we recommend the unconjugated version of this antibody, 66678-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL594-66678 targets PSMA/GCPII in IF, FC (Intra) applications and shows reactivity with Human, Rat samples.

Tested Reactivity Human, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PSMA/GCPII fusion protein Ag16594 相同性解析による交差性が予測される生物種
Full Name folate hydrolase (prostate-specific membrane antigen) 1
Calculated molecular weight 719 aa, 81 kDa
Observed molecular weight 100-120 kDa
GenBank accession numberBC025672
Gene symbol FOLH1
Gene ID (NCBI) 2346
RRIDAB_2920020
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PSMA(Prostate-specific membrane antigen) is also named as FOLH1, FOLH, NAALAD1, PSM and belongs to the peptidase M28 family. PSMA is a 100-120 kDa integral transmembrane glycoprotein, considered to be a highly specific marker of the prostate gland, and has successfully been used as a marker of circulating prostatic epithelial cells(PMID:10074909; 15680901). It is involved in conversion of the major neurotransmitter (NAAG) to NAA and free glutamate. It has 8 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
IF protocol for CL594 PSMA/GCPII antibody CL594-66678Download protocol
Standard Protocols
Click here to view our Standard Protocols