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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PSD95 Polyclonal antibody

PSD95 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30255-1-AP

Synonyms

Disks large homolog 4, DLG4, FLJ97752, FLJ98574, PSD 95, PSD95, SAP 90, SAP90, Synapse associated protein 90

Validation Data Gallery

Applications:
  • WB analysis using 30255-1-AP

    WB analysis using 30255-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30255-1-AP (PSD95 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 30255-1-AP

    IHC staining of mouse brain using 30255-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30255-1-AP (PSD95 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

30255-1-AP targets PSD95 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSD95 fusion protein Ag33000 相同性解析による交差性が予測される生物種
Full Name discs, large homolog 4 (Drosophila)
Calculated molecular weight81 kDa
Observed molecular weight90-95 kDa
GenBank accession numberNM_001365
Gene symbol DLG4
Gene ID (NCBI) 1742
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PSD-95 (postsynaptic density protein 95) also known as SAP-90 (synapse-associated protein 90) is a protein that in humans is encoded by the DLG4 (disks large homolog 4) gene. PSD-95 is a scaffolding protein of the MAGUK protein family, and engages in several vital protein-protein interactions in the brain with its PDZ domains. It has been suggested that PSD-95 is composed of two supramodules, one of which is the PDZ1-2 tandem domain. It plays an important role in synaptic plasticity and the stabilization of synaptic changes during long-term potentiation. Observed MW of PSD95 is 90-95 kDa due to phosphorylation (PMID: 20682303).

Protocols

Product Specific Protocols
WB protocol for PSD95 antibody 30255-1-APDownload protocol
IHC protocol for PSD95 antibody 30255-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols