Various lysates were subjected to SDS PAGE followed by western blot with 10664-1-AP (PRDX3 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

HeLa cells were subjected to SDS PAGE followed by western blot with 10664-1-AP (PRDX3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

WB result of PRDX3 antibody (10664-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRDX3 transfected HEK-293 cells.

MCF-7 cells were subjected to SDS PAGE followed by western blot with 10664-1-AP (PRDX3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 10664-1-AP (PRDX3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

WB result from Qing-Xi Yue, (PMID:20449493); “Control” and “GAD-treated” above the panel represent the control cells and HeLa cells treated with 10 μMGAD for 48 h, respectively. Each blot is the representative result of three independent experiments.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10664-1-AP (PRDX3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10664-1-AP (PRDX3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRDX3 antibody (10664-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).