Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse brain tissue, mouse hippocampal tissue |
| Positive IF/ICC detected in | transfected cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IF | See 1 publications below |
Product Information
17261-1-AP targets SynDIG4/PRRT1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SynDIG4/PRRT1 fusion protein Ag10958 相同性解析による交差性が予測される生物種 |
| Full Name | proline-rich transmembrane protein 1 |
| Calculated molecular weight | 225aa,23 kDa; 306aa,31 kDa |
| Observed molecular weight | 35-40 kDa |
| GenBank accession number | BC013201 |
| Gene Symbol | SynDIG4 |
| Gene ID (NCBI) | 80863 |
| RRID | AB_2878371 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99946 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SynDIG4 [also known as Prrt1 (proline-rich transmembrane protein 1)] has recently been identified as a component of AMPAR complexes. SynDIG4 is especially prominent in the hippocampus and particularly within CA1.The function of PRRT1 has not been widely studied, and is yet to be fully elucidated.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SynDIG4/PRRT1 antibody 17261-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Synaptic Neurosci Interaction and Subcellular Association of PRRT1/SynDIG4 With AMPA Receptors. | ||
Mol Cell Neurosci PRRT1 regulates basal and plasticity-induced AMPA receptor trafficking. | ||
Zoolog Sci A Calaxin Gene in the Pacific Oyster Crassostrea gigasand Its Potential Roles in Cilia. | ||
Alzheimers Res Ther Uncovering the epigenetic regulatory clues of PRRT1 in Alzheimer's disease: a strategy integrating multi-omics analysis with explainable machine learning
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