Validation Data Gallery

Filter: AllWBIHC

WB analysis of mouse kidney using 15414-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15414-1-AP (PRPF19 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat kidney using 15414-1-AP
rat kidney tissue were subjected to SDS PAGE followed by western blot with 15414-1-AP (PRPF19 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 15414-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 15414-1-AP (PRPF19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15414-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15414-1-AP (PRPF19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 15414-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 15414-1-AP (PRPF19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 15414-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 15414-1-AP (PRPF19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 15414-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 15414-1-AP (PRPF19 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human stomach using 15414-1-AP
Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 15414-1-AP (PRPF19 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PRPF19 Polyclonal antibody

PRPF19 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15414-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Synonyms

EC:2.3.2.27, hPso4, Pre-mRNA-processing factor 19, PRP19, PSO4

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (6)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse kidney tissue, HEK-293 cells, HeLa cells, human testis tissue, mouse liver tissue, PC-3 cells, rat kidney tissue
Positive IHC detected in	human stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 6 publications below

Product Information

15414-1-AP targets PRPF19 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7700 Product name: Recombinant human PRPF19 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 155-504 aa of BC008719 Sequence: GAGEPMDLGELVGMTPEIIQKLQDKATVLTTERKKRGKTVPEELVKPEELSKYRQVASHVGLHSASIPGILALDLCPSDTNKILTGGADKNVVVFDKSSEQILATLKGHTKKVTSVVFHPSQDLVFSASPDATIRIWSVPNASCVQVVRAHESAVTGLSLHATGDYLLSSSDDQYWAFSDIQTGRVLTKVTDETSGCSLTCAQFHPDGLIFGTGTMDSQIKIWDLKERTNVANFPGHSGPITSIAFSENGYYLATAADDSSVKLWDLRKLKNFKTLQLDNNFEVKSLIFDQSGTYLALGGTDVQIYICKQWTEILHFTEHSGLTTGVAFGHHAKFIASTGMDRSLKFYSL 相同性解析による交差性が予測される生物種
Full Name	PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae)
Calculated molecular weight	55 kDa
Observed molecular weight	55 kDa
GenBank accession number	BC008719
Gene Symbol	PRPF19
Gene ID (NCBI)	27339
RRID	AB_10637855
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UMS4
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PRPF19 is involved in several processes, including DNA damage checkpoint signaling; cellular protein metabolic process; and mRNA splicing, via spliceosome.

Protocols

Product Specific Protocols
IHC protocol for PRPF19 antibody 15414-1-AP	Download protocol
WB protocol for PRPF19 antibody 15414-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Expert Opin Ther Targets Proteome of thymus and spleen reveals that 10-hydroxydec-2-enoic acid could enhance immunity in mice. Authors - Pei Fan View Article
human	WB	Arch Biochem Biophys RNF113A as a Poor Prognostic Factor Promotes Metastasis and Invasion of Cervical Cancer through miR197/PRP19/P38MAPK Signaling Pathway Authors - Qingwei Zhang View Article
human	WB	J Cell Biochem Human RNF113A participates of pre-mRNA splicing in vitro. Authors - Guilherme H Gatti da Silva View Article
human	WB	iScience RRM2 promotes liver metastasis of pancreatic cancer by stabilizing YBX1 and activating the TGF-beta pathway Authors - Zhouyuan Du KD Validated View Article
mouse	WB	iScience Adipocyte-specific Steap4 deficiency reduced thermogenesis and energy expenditure in mice Authors - Han Wang View Article
pig,human	WB	J Virol PRPF19 Limits Porcine Epidemic Diarrhea Virus Replication through Targeting and Degrading Viral Capsid Protein Authors - Xueying Zhai View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse kidney using 15414-1-AP

WB analysis of rat kidney using 15414-1-AP

WB analysis of HEK-293 using 15414-1-AP

WB analysis of HeLa using 15414-1-AP

WB analysis of human testis using 15414-1-AP

WB analysis of PC-3 using 15414-1-AP

WB analysis of mouse liver using 15414-1-AP

IHC staining of human stomach using 15414-1-AP

PRPF19 Polyclonal antibody

PRPF19 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Proteome of thymus and spleen reveals that 10-hydroxydec-2-enoic acid could enhance immunity in mice.

RNF113A as a Poor Prognostic Factor Promotes Metastasis and Invasion of Cervical Cancer through miR197/PRP19/P38MAPK Signaling Pathway

Human RNF113A participates of pre-mRNA splicing in vitro.

RRM2 promotes liver metastasis of pancreatic cancer by stabilizing YBX1 and activating the TGF-beta pathway

Adipocyte-specific Steap4 deficiency reduced thermogenesis and energy expenditure in mice

PRPF19 Limits Porcine Epidemic Diarrhea Virus Replication through Targeting and Degrading Viral Capsid Protein