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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PRKAR2A Monoclonal antibody

PRKAR2A Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1B2B3

Cat no : 67751-1-Ig

Synonyms

PKR2, PRKAR2, PRKAR2A

Validation Data Gallery

Applications:
  • WB analysis using 67751-1-Ig

    WB analysis using 67751-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67751-1-Ig (PRKAR2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 67751-1-Ig

    IHC staining of human colon cancer using 67751-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67751-1-Ig (PRKAR2A antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PRKAR2A antibody included
  • IHC staining of human colon cancer using 67751-1-Ig

    IHC staining of human colon cancer using 67751-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67751-1-Ig (PRKAR2A antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PRKAR2A antibody included
  • IHC staining of mouse colon using 67751-1-Ig

    IHC staining of mouse colon using 67751-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 67751-1-Ig (PRKAR2A antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PRKAR2A antibody included
  • IHC staining of mouse colon using 67751-1-Ig

    IHC staining of mouse colon using 67751-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 67751-1-Ig (PRKAR2A antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PRKAR2A antibody included
  • IF Staining of HeLa using 67751-1-Ig

    IF Staining of HeLa using 67751-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PRKAR2A antibody (67751-1-Ig, Clone: 1B2B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inU2OS cells, NIH/3T3 cells, HSC-T6 cells, Jurkat cells, HepG2 cells, HeLa cells, LNCaP cells
Positive IHC detected inhuman colon cancer tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PRKAR2A Ready-To-Use IHC Kit. PRKAR2A primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

67751-1-Ig targets PRKAR2A in WB, IF, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PRKAR2A fusion protein Ag29893 相同性解析による交差性が予測される生物種
Full Name protein kinase, cAMP-dependent, regulatory, type II, alpha
Calculated molecular weight 43 kDa
Observed molecular weight50 kDa
GenBank accession numberBC002763
Gene symbol PRKAR2A
Gene ID (NCBI) 5576
RRIDAB_2918520
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The effects of cAMP in most tissues and cell types are mainly modulated via protein kinase A, a heterotetrameric protein complex consisting of two regulatory and two catalytic subunits. The regulatory subunit of cAMP-dependent protein kinase (PRKAR2A) is one of the regulatory subunits and the gene is located on chromosome region 3p21.3-p21.2. The expression of PRKAR2A is tightly regulated during spermatogenesis, a significant increase in expression of this gene was also found in the human myometrium during pregnancy.

Protocols

Product Specific Protocols
WB protocol for PRKAR2A antibody 67751-1-IgDownload protocol
IHC protocol for PRKAR2A antibody 67751-1-IgDownload protocol
IF protocol for PRKAR2A antibody 67751-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols