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  • Featured Product
  • KD/KO Validated

PRKACA Polyclonal antibody

PRKACA Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24503-1-AP

Synonyms

PKA C alpha, PKACA, PRKACA

Validation Data Gallery

Applications:
  • WB analysis using 24503-1-AP

    WB analysis using 24503-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24503-1-AP (PRKACA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 24503-1-AP

    WB analysis of HeLa using 24503-1-AP

    WB result of PRKACA antibody (24503-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRKACA transfected HeLa cells.

  • IHC staining of human breast cancer using 24503-1-AP

    IHC staining of human breast cancer using 24503-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24503-1-AP (PRKACA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PRKACA antibody included
  • IHC staining of human breast cancer using 24503-1-AP

    IHC staining of human breast cancer using 24503-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24503-1-AP (PRKACA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PRKACA antibody included

Tested Applications

Positive WB detected inNeuro-2a cells, HeLa cells, SH-SY5Y cells, mouse testis tissue, rat testis tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PRKACA Ready-To-Use IHC Kit. PRKACA primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 24503-1-AP is PRKACA Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRKACA fusion protein Ag21478 相同性解析による交差性が予測される生物種
Full Name protein kinase, cAMP-dependent, catalytic, alpha
Calculated molecular weight 41 kDa
Observed molecular weight 38-43 kDa
GenBank accession numberBC039846
Gene symbol PRKACA
Gene ID (NCBI) 5566
RRIDAB_2879578
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PRKACA antibody 24503-1-APDownload protocol
IHC protocol for PRKACA antibody 24503-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Front Pharmacol

Integrating network pharmacology and experimental verification to explore the mechanism of puerarin against oliguria in acute alcoholism

Authors - Mei-Xuan Wan
View Article