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  • KD/KO Validated

AMPK Beta 1 Polyclonal antibody

AMPK Beta 1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10308-1-AP

Synonyms

AMPK, AMPK beta 1, AMPK subunit beta 1, AMPKb, HAMPKb, PRKAB1



Tested Applications

Positive WB detected inmouse liver tissue, A431 cells, HEK-293 cells, HeLa cells
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10308-1-AP targets AMPK Beta 1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AMPK Beta 1 fusion protein Ag0301 相同性解析による交差性が予測される生物種
Full Name protein kinase, AMP-activated, beta 1 non-catalytic subunit
Calculated molecular weight 38 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC001007
Gene symbol PRKAB1
Gene ID (NCBI) 5564
RRIDAB_513239
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for AMPK Beta 1 antibody 10308-1-APDownload protocol
IHC protocol for AMPK Beta 1 antibody 10308-1-APDownload protocol
IF protocol for AMPK Beta 1 antibody 10308-1-APDownload protocol
IP protocol for AMPK Beta 1 antibody 10308-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Cell Metab

CircACC1 Regulates Assembly and Activation of AMPK Complex under Metabolic Stress.

Authors - Qidong Li
  • KD Validated
mouseWB

Acta Pharm Sin B

Engineering cannabidiol synergistic carbon monoxide nanocomplexes to enhance cancer therapy via excessive autophagy

Authors - Chang Xiao
mouseWB,IF

Theranostics

Therapeutic inhibition of miR-802 protects against obesity through AMPK-mediated regulation of hepatic lipid metabolism.

Authors - Yangyue Ni
human,mouseWB

J Ethnopharmacol

Mulberry leaves attenuate D-galactose-induced aging in vivo and in vitro

Authors - Yan Zhu
humanWB

Hum Cell

RNF126 contributes to stem cell-like properties and metastasis in hepatocellular carcinoma through ubiquitination and degradation of LKB1

Authors - Jie Huang
ratWB

Dig Dis Sci

Heme Oxygenase-1-Modified BMMSCs Activate AMPK-Nrf2-FTH1 to Reduce Severe Steatotic Liver Ischemia-Reperfusion Injury

Authors - Xiaorong Tian