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peroxiredoxin 2 Polyclonal antibody

peroxiredoxin 2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Cat no : 10545-2-AP

Synonyms

PRDX2, Peroxiredoxin-2, NKEF-B, NKEFB, Natural killer cell-enhancing factor B


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Tested Applications

Positive WB detected inHepG2 cells, C2C12 cells, PC-12 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver tissue, human hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:600-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10545-2-AP targets peroxiredoxin 2 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen peroxiredoxin 2 fusion protein Ag0835 相同性解析による交差性が予測される生物種
Full Name peroxiredoxin 2
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC003022
Gene symbol peroxiredoxin 2
Gene ID (NCBI) 7001
RRIDAB_2168202
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PRDX2, also named as TSA, PRP, NKEFB and TDPX1, belongs to the ahpC/TSA family. It is known to act as an antioxidant enzyme whose main function is H2O2 reduction in cells. PRDX2 is involved in redox regulation of the cell. It reduces peroxides with reducing equivalents provided through the thioredoxin system. It may play an important role in eliminating peroxides generated during metabolism. PRDX2 might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2. PRDX2 actions may be related to the expression of NFKB and IKB.(PMID:21248284)

Protocols

Product Specific Protocols
WB protocol for peroxiredoxin 2 antibody 10545-2-APDownload protocol
IHC protocol for peroxiredoxin 2 antibody 10545-2-APDownload protocol
IF protocol for peroxiredoxin 2 antibody 10545-2-APDownload protocol
IP protocol for peroxiredoxin 2 antibody 10545-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

ACS Nano

Engineering Extracellular Vesicles Restore the Impaired Cellular Uptake and Attenuate Intervertebral Disc Degeneration.

Authors - Zhiwei Liao
ratWB

Br J Pharmacol

Luteolin ameliorates rat myocardial ischemia-reperfusion injury through peroxiredoxin II activation.

Authors - Bo Wei
humanWB, IHC

Oncogene

PMID: 26500057

Authors - Y-H Park
human,mouseWB,IP,CoIP

Free Radic Biol Med

Oxidation resistance 1 regulates post-translational modifications of peroxiredoxin 2 in the cerebellum.

Authors - Daria M Svistunova
mouseIF

Stem Cells

Dominant role of peroxiredoxin/JNK axis in stemness regulation during neurogenesis from embryonic stem cells.

Authors - Sun-Uk Kim
humanWB,IHC

Oxid Med Cell Longev

Azelaic Acid Exerts Antileukemia Effects against Acute Myeloid Leukemia by Regulating the Prdxs/ROS Signaling Pathway.

Authors - Dongdong Zhang