Validation Data Gallery

Filter: AllWBIHC

WB analysis of mouse testis using 19653-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 19653-1-AP (PPP3CC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 19653-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 19653-1-AP (PPP3CC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 19653-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 19653-1-AP (PPP3CC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 19653-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 19653-1-AP (PPP3CC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 19653-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19653-1-AP (PPP3CC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 19653-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 19653-1-AP (PPP3CC Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human stomach using 19653-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 19653-1-AP (PPP3CC antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PPP3CC Polyclonal antibody

PPP3CC Polyclonal Antibody for WB, IHC, ELISA

Cat No. 19653-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

CNA3, CAM-PRP catalytic subunit, CAM PRP catalytic subunit, CALNA3, Calmodulin-dependent calcineurin A subunit gamma isoform

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse testis tissue, human testis tissue, Jurkat cells, MCF-7 cells, mouse brain tissue, mouse skeletal muscle tissue
Positive IHC detected in	human stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below

Product Information

19653-1-AP targets PPP3CC in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7619 Product name: Recombinant human PPP3CC protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 153-512 aa of BC004864 Sequence: TDYFTFKQECRIKYSEQVYDACMETFDCLPLAALLNQQFLCVHGGMSPEITSLDDIRKLDRFTEPPAFGPVCDLLWSDPSEDYGNEKTLEHYTHNTVRGCSYFYSYPAVCEFLQNNNLLSIIRAHEAQDAGYRMYRKSQATGFPSLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVGEKVTEMLVNVLNICSDDELISDDEAEGSTTVRKEIIRNKIRAIGKMARVFSILRQESESVLTLKGLTPTGTLPLGVLSGGKQTIETATVEAVEAREAIRGFSLQHKIRSFEEARGLDRINERMPPRKDSIHAGGPMKSVTSAHSHAAHRSDQGKKAHS 相同性解析による交差性が予測される生物種
Full Name	protein phosphatase 3 (formerly 2B), catalytic subunit, gamma isoform
Calculated molecular weight	57 kDa
Observed molecular weight	57 kDa
GenBank accession number	BC004864
Gene Symbol	PPP3CC
Gene ID (NCBI)	5533
RRID	AB_10695630
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P48454
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IHC protocol for PPP3CC antibody 19653-1-AP	Download protocol
WB protocol for PPP3CC antibody 19653-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	FASEB J Roles of glycogen synthase kinase 3 alpha and calcineurin in regulating the ability of sperm to fertilize eggs. Authors - Souvik Dey View Article
human	WB	BMC Complement Med Ther Shikonin reactivates TSGs GADD45B and PPP3CC to block NSCLC cell proliferation and migration through JNK/P38/MAPK signaling pathways Authors - Yujia Zhao View Article

製品

アプリケーション

cGMPグレード製品

サポート

カスタム対応

プロモーション

企業情報

抗体

免疫アッセイ

サイトカイン・増殖因子

ChromoTek VHH（Nanobody®）試薬

サポートツール・試薬

ゲノミクス試薬

研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse testis using 19653-1-AP

WB analysis of mouse brain using 19653-1-AP

WB analysis of MCF-7 using 19653-1-AP

WB analysis of human testis using 19653-1-AP

WB analysis of mouse skeletal muscle using 19653-1-AP

WB analysis of Jurkat using 19653-1-AP

IHC staining of human stomach using 19653-1-AP